How we can dissolve theophylline derivatives for the MTT test?

I have a question about your letter, Fluorescent phosphoinositide 3-kinase inhibitors suitable for monitoring of intracellular distribution. Can you tell me how we can dissolve theophylline for...

26 January 2021 9,094 1 View

Can anybody suggest me a cheap material for surface modification of hydrophilic Titania nanoparticles?

I'm looking for a cheap material to modify Titania nanoparticles in order to use them in polymer and increase its contact angle.

13 December 2020 504 5 View

Importance of the tunnel effect on the solar cells performance?

Does the tunnel effect play a major role in the performance of solar cells? Especially when the device is heavily doped so the depletion layer can be thin enough for tunneling. Are there any...

10 December 2020 5,518 10 View

In XPS analysis of GO films. How I can use the sample work function method to know the information of XPS binding energy scale is referenced ?

Note that aligning the spectra to the C 1s peak of adventitious carbon is a highly risky procedure and was recently shown to lead to unphysical results. Therefore, I strongly suggest to avoid it....

18 November 2020 5,483 4 View

Dear Doctors/Professors, i would like to ask if a novel drug would be tested in animal model in mice which range of doses do i use first ?

Dear Doctors/Professors, i would like to ask if a novel drug would be tested in animal model in mice which range of doses do i use first ?

03 August 2020 3,811 3 View

How can I find a questionnaire for Corporate identity?

My thesis is about the relation between corporate social responsibility and corporate identity. And I can not find any questionnaire about it. Can you help me?

24 July 2020 3,507 3 View

When I injected it into the LCMS all the chromatogram peaks are small lthough I have increased the concentration?

I have a metabolic extract from a plant, when I injected it into the LCMS all the chromatogram peaks are small, although I have increased the concentration several times but there is no change in...

12 March 2020 3,047 2 View

How to deal with a skewed Likert scale data with Ceiling/Flooring effects in a before-after design?

I have data of 30 subjects from a before-after design study. We used likert scale to investigate effects of changes in mood on some dependent variables. We hypothesized that the intervention would...

22 February 2020 6,496 5 View

What are the quantities per microliter of the different components needed for a reaction (1isolate of S. aureus ) to run a multiplex PCR ?

Dear colleagues, I need help. I want to run a multiplex PCR to look for enterotoxin-encoding genes in coagulase-positive StaphyloccIoccu . I have all the reagents needed to prepare the MIx PCR...

13 February 2020 7,036 2 View

How can I do the Magnetic calculations using CASTEP code as ferromagnetic system , anti-ferromagnetic system ?

with a CASTEP

01 February 2020 1,612 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View