Leila Abdelhamid

6 Questions 7 Answers 0 Followers

Questions related from Leila Abdelhamid

How to improve detection of phosphorylation target on Western blot on immunoprecipitated protein?

Hi All, I am trying to detect phosphorylation of a protein in cell lysates, I do immunoprecipitation of the target protein then look for Pan-phosphotyrosine by western blot. I did precipitate the...

16 May 2022 2,687 0 View

RNA extraction from paraffin embedded tissue ?

Hi All, I have paraffin embedded mice joints. I need to extract RNA however, the samples went through decalcification using EDF (formic acid, formaldehyde and methanol ready to use reagent). So,...

13 February 2019 9,391 2 View

Can mineral oil interfere with fluorescence reporting in RT-PCR?

Hi all, I am doing reverse transcription for my RNA isolate to proceed to RT-PCR using such cDNA products. we have a non heated lid thermal cycler, so I have to add some mineral oil on my RT mix...

03 June 2016 2,147 4 View

Any Suggestions about RNA degradation?

Hi all, I need to determine the concentration of my isolated RNA, but we have no nanodrop machine in our facility. So, I have to travel about 2 hours for measuring it. The problem is how to...

17 April 2016 10,112 7 View

Can I use eppendrof safe-lock 0.5ml tubes for MX3000 P qPCR machine?

I want to run PCR on MX3000P qPCR machine from Agilent for the first time! so can I use eppendorf safe-lock 0.5ml PCR tubes ( available ) or I have to buy the Agilent optical tubes? I am...

18 March 2016 6,590 3 View

Any suggestions about RNA gel electrophoresis?

I am planning to run total RNA following Tizol extraction on non-denature gel ( from my reading I think it will be ok if I run on 1% agrose agel in 0.5 x TBE and using lower voltage and minimal...

14 March 2016 5,256 11 View