We recently purchased a liquid nitrogen storage freezer. Up until now, we have stored all our cells at -80C. I have stocks that have been stored at -80C for 6-8 months. Does anyone know if the change in temperature from -80C to liquid nitrogen could adversely affect cell viability?
It should be no problem.
It's standard procedure to first slowly free cells in a -80degC freezer, using a foam or a Mr. Frosty type IPA container overnight (at least) and then move them to liquid nitrogen for long term storage.
You could always take a tube and test it before moving the rest of your stocks -- generally a good idea regardless.
Thanks. I wasn't sure if after long-term storage at -80, transferring to liquid nitrogen would be a problem. I will test a tube or two - that's a good idea. Thanks again.
If you plan on using the cells quickly, then it doesn't matter too much, but remember, -80C is not cold enough for truly long term storage and over time damage/loss of viability is likely to occur. Transferring the vials to LN2 of course will not reverse this, just halt it.
Now, the definition of "long term" varies with cell type and specific conditions, but I would say 6-12 months is pushing it. If you think you need to store those cells longer, I would certainly get those cells in LN2.
Can I transfer cells to liquid nitrogen after 6-8 months of storage at -80C?
-80 c is not for long term storage of the cells, however it will be ok to transfer your cells to liquid nitrogen after 6-8 months storage at -80 c.
Regards
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