I know steam has become a potential for killing invasive plants. I am curious to know whether or not it has been used successfully in killing the plant and the seeds of Mile-a-minute (Persicaria perfoliata).
What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?
12 October 2020 433 5 View
I'm digesting a few different mammalian tissues with a spin kit and proteinase k. Some tissues (esp. testis and pituitary gland) look fully digested and clear. But once the lysis buffer is added,...
09 August 2020 4,443 2 View
I'm extracting DNA from various bull tissues and there are some that don't work with a spin column kit, like brain tissue that contains a lot of lipids. For those, we do Phenol-chloroform...
05 August 2020 2,126 2 View
Hello! I would like to understand in what cases blood or some other biological liquid passes through implant or graft with some porous microstructure according the Darcy law (thanks to the...
26 March 2020 8,670 14 View
Hi Is anyone aware of a publication or article discussing the convergent validity or crosswalk of the K10 and PHQ9 for a measure of depression? Many thanks
03 March 2020 8,601 1 View
why most people use chitosan tpp instead of alginat-cacl2 as a nanoencapsulation?
13 February 2020 251 3 View
I found literature of and about J. O. Crites' Career Maturity Inventory. I cannot find the inventory tool /items. May someone shares their experiences and ideas on this topic with me? Which...
11 February 2020 1,387 3 View
We have used TransIT-X2 (Mirus) for years to transfect different cell lines, but suddenly I am having a lot of trouble with my HEK 293 MSR cells becoming very round and dying/detaching soon after...
07 February 2020 6,375 3 View
I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...
12 November 2019 9,677 5 View
Should postgraduate students be required to transcribe research interviews or can they be allowed to analyse their aural (recorded) data? I have a postgraduate student who wants to analyse her...
24 October 2019 2,006 6 View
Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...
26 February 2021 10,014 3 View
Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...
25 February 2021 2,442 1 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...
22 February 2021 7,127 4 View
Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...
17 February 2021 1,434 13 View
biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...
15 February 2021 1,102 5 View
Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...
11 February 2021 6,683 6 View
1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.
04 February 2021 9,069 3 View
I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...
04 February 2021 9,152 4 View
I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...
03 February 2021 4,867 3 View