Previously i stored my glycerol stock of bacterial culture at -20 degree. but now its under repair so for sometime can i transfer the bacterial stock in -80? or any chances of mutation of bacteria from this transfer?
Dear Anupam
In short yes you can. In fact in the past I routinely stored at -80C and not -20C
Dear Anupam,
Glycerol stocks should be stored in -80, preferably.
Go ahead.
Yes! -80 is ideal for long term storage. What % glycerol are you using? We use 30% for -80 stocks.
Of course you can!!
In a huge number of cases the volume and shape of ice crystals is more compatible with biological molecules and membranes at -80 rather than -20.
I keep duplicate stocks of all bacterial cultures. The ones at -20 are the working cultures; the ones at -80 are archived in case the working culture dies, becomes contaminated, freezer failure, ...
I agree with the others that -80C is the preferred storage temp for glycerol stocks. Also, you will have no concerns about stability when you transfer them. They are quite robust. I was even in a lab once where the -80C failed and all of our glycerol stocks thawed and remained thawed for a day or two before we found them. We moved them to a different -80C and they were all viable. (Though, every time I grew a culture from the thawed set, I checked the sequence and froze a fresh glycerol stock).
Yes storage at minus 80 is better as recommended by many on this forumAnother advice
If it is strains for isolation of plasmids then minus 80 is better while for strains for expression of proteins - storing the DNA in minus 20 is better
Minus 80 is always preferred over minus 20 especially for flash freezing of samples for example competent cell preparation
Hope this helps
Actually lower the temperature of storage of bacterial culture longer the life of culture will be. Almost everybody keep glycerol stocks at -80°C. Storage at -20°C is being done only under conditions of no -80°C freezer available.
Even one can store it in liquid nitrogen, but it would expensive.
Bacteria can be keep in LB agar plate at 4°C for a some weeks. However, if you want to store bacteria for a longer time, you will need to establish glycerol stocks, because the glycerol can stabilize bacteria and prevent the damage of the cell. And you can stored it at -80°C for many years.
I have prepared 20% glycerol stock of bacterial culture and preserved at -80 °C, my culture was revived easily. So yes, according to my experience you can store 20% glycerol stock in -80.
Yes you can.. Because gycerol protect the bacterial cell surface to be damaged. You can store the stock in -70 or -80 but it is good to make new culture in every 6 months for getting healthy cells.
Yes. Glycerol stocks can be stored in - 80 degrees for approx 6-7 months without any problem. Make sure of the stock concentration. It affects the efficiency of the same.
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