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Questions related from Anupam Apoorva
Dear all, I have a compound with molecular formula C20H29O3, the predicted m/z value from ChemDraw is 153.092 (100%), however, my real high-resolution mass spectrometry results shown 153.055 m/z...
11 March 2021 3,607 1 View
Hello Everyone, I have made the polymeric solution of two different polymers. My aim involves preparation of beads from this polymeric solution and encapsulation of my drug to these beads that I...
27 August 2018 7,114 11 View
Hello, I have to prepare polyphenolics loaded chitosan nanoparticle. so, what mw of chitosan should i select to get the less particle size with good zeta value and consistent drug release.
15 March 2017 7,120 3 View
Hello , I am making plant polyphenol loaded chitosan nanoparticle. I took chitosan and TPP in 3 :1 ratio. so, total reaction mixture includes 6ml chitosan and 2 ml TPP. with control i got the...
10 October 2016 4,632 14 View
Hello , i am doing catalase assay for plant extract. The reaction mixture contains (2.5ml) contains 1ml of 50mMphosphate buffer(pH 7.0) , 0.5ml of 0.5mM H2O2 and 1ml enzyme extract of...
27 September 2016 3,364 5 View
To calculate the cfu discrete colonies are required to get that gives the information that how many microbial cells we are going to inoculate into the plate. but for study of plates suppose for...
25 June 2016 8,976 7 View
I want to do antioxidant enzymatic assay of plant sample. For that will i have to do the assay of pure enzymes also (standard) for each enzymatic assay? How to calculate the enzyme concentration...
20 May 2016 4,959 2 View
I am planning to start antioxidant enzymatic assay of spinach plant. i will do this assay from the stem part of the plant. Can anyone suggest me the standard protocol for this assay Waiting for...
17 May 2016 5,889 2 View
Previously i stored my glycerol stock of bacterial culture at -20 degree. but now its under repair so for sometime can i transfer the bacterial stock in -80? or any chances of mutation of bacteria...
17 May 2016 10,036 23 View
I am doing antibacterial testing by disc diffusion method for nanofibre film which exhibits antibacterial activity that has been already reported in many literatures. on same bacterial plate...
30 April 2016 8,102 6 View
My plant extract is in 70% ethanol. I am using 100%methanol for preparing the plant stock solution as well as DPPH stock solution. i am getting increased absorbance for my plant extracts. So,...
29 February 2016 1,154 1 View
I am doing MTTassay against bone cancer cell line . My test compound is crude plant extract rich in polyphenols which are antioxidant in nature. I took the plant sample concentration in...
23 December 2015 5,827 5 View
I am doing MTT assay for my plant extract. after cell seeding how should i dilute my plant extract for better result. should i dilute it in that solvent in which it has been prepared or in cell...
22 December 2015 9,190 2 View
