Laurence Stuart Dawkins-Hall

6 Questions 618 Answers 0 Followers

Questions related from Laurence Stuart Dawkins-Hall

Transwell Migration assay ?

Dear Colleagues I am bout to explore the extravasation of tumours by in vitro modelling using a trans well (Boyden chamber) assay; I must confess this is completely new to me In essence, we are...

08 May 2017 8,463 3 View

Standard curves in qPCR for very low copy number genes ?

Dear Colleague(s) I am dealing with a gene at the moment that exhibits very low expression and is barely detectable from neat cDNA. As such if you dilute the cDNA to construct a standard curve...

02 June 2016 4,322 7 View

REST Gene expression software: Windows compatibility issues ?

Dear Colleague(s) I am trying to run REST 2009 on a PC platform with windows 7 OS; It doesn't appear to want to run Are there some compatibility issues ? Am I attempting to install and run the...

24 March 2016 6,611 6 View

Housekeeper choice for real time qPCR using human heart RNA ?

Dear Colleague(s) I am well versed with real time qPCR but have never actually worked with human heart tissue to determine relative expression of a GOI, viz cytochrome p450 2J2 variant. In...

15 January 2016 3,113 2 View

Does anyone know if you can perform delta delta Ct analysis on gel based bands ?

Hi, I routinely measure gene expression by qPCR using the delta delta ct method, wherein a test gene is measured in treated and untreated samples and both are normalised (for loading/amplification...

21 October 2014 5,677 7 View

Binding of DNA to histone core octamer: SNP's and effects on binding ?

Dear Colleague(s) I am not a histone biologist but have been asked to contemplate the feasibility - indeed sensibility - of a histone binding assay: In essence we are proposing to look at the...

01 January 1970 7,533 3 View