Mark K Chee

10 Questions 172 Answers 0 Followers

Questions related from Mark K Chee

Can red fluorescent protein be used as a chromoprotein?

More than one person has observed that accumulation of red fluorescent protein leads to a deep pink color that is visible to the naked eye without fluorescence imaging. Can it thus be quantified...

02 June 2025 7,197 1 View

How to perform LAP (leucine aminopeptidase) assay for bacteria without using LAP disks?

I am unable to find a convenient source for LAP disks at the moment and would like to instead purchase the substrate (leucine ß-naphthylamide) found on these disks instead. Can anyone tell me what...

23 January 2022 6,237 0 View

Gram-positive bacteria inhibited by sodium azide?

We are characterizing a novel gram-positive bacterium and noticed an unexpected phenotype. This bacterium fails to form colonies on BHIA with 0.025% sodium azide even though it is facultatively...

10 October 2021 6,335 1 View

What plasmids can I use for fluorescent labeling of lactic acid bacteria?

I am interested in labeling Lactobacillus plantarum with either a red (preferably) or a green fluorescent protein. Can anyone recommend a paper that describes a molecular tool for doing this?...

23 June 2021 3,266 4 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

11 February 2021 6,382 1 View

Do you know where to obtain the Bio-Rad MyCycler Gradient Upgrade Kit #1709707EDU?

Hi everyone, I am looking to obtain a copy of the CD-ROM and an activation key that is part of this upgrade kit. We have a MyCycler at our college that has not been upgraded with the temperature...

15 September 2017 7,981 3 View

Is store-bought glycerin (glycerol) USP 99.5% suitable for use as a bacterial cryoprotectant?

It's my guess that this is no different from the glycerol sold by lab supply companies but I was curious to see if anyone has tried using store-bought glycerin to freeze bacteria for cryo-storage...

08 September 2017 4,141 4 View

Requirements for triggering endosporulation in Paenibacillus species?

Does anyone have any insight into the endosporulation requirements for Paenibacillus species apart from Paenibacillus larvae? Thank you.

11 June 2016 5,138 2 View

Does anyone know how to induce sporulation in Lysinibacillus fusiformis, formerly Bacillus fusiformis?

The sporulation requirements of this species seem to differ from those of the closely related Lysinibacillus sphaericus. I have tried using a sporulation salt medium published to work with the...

15 October 2015 6,391 8 View

Does anyone have any information on serum requirements for Drosophila S2 cells?

I would like to find out if anyone has attempted to maintain S2 cell cultures using Schneider's medium supplemented with calf serum or newborn calf serum, which I've found are cheaper alternatives...

14 August 2014 7,636 9 View