Gautam Krishnan

6 Questions 18 Answers 0 Followers

Questions related from Gautam Krishnan

Hello!I have a monocytic cell line THP-1 .Can I use it to do cytokine upregulation studies without adding PMA.?

I am trying to simulate an in vivo situation in host macrophages by adding a recombinant protein that is expressed in high amounts.Can i do cytokine upregulation studies without adding PMA ?Will...

12 December 2017 4,438 2 View

How to use NATIVE PAGE to determine the oligomeric status of a protein ?

The recombinant protein 18kDA has been purified by His Tag and also run on a gel filtration column where it appears in the void and comparing with standards appears between 158000 Kda and 67000...

10 August 2017 6,632 5 View

How can I identify His tag protein when the level of induction is poor?

I have expressed a protein of around 18Kda with C terminal his tag in pET21A.I have very low level of expression in pellet with protein going in pellet.I did low temperature expression to get...

04 February 2016 7,634 2 View

What is the minimum amount of protein that can be easily visualised on PAGE by coomassie blue staining and silver staining?

I have a protein that barely shows up on SDS PAGE and i want to visualise it on Silver stain.How much do i need to load for it to be visible

29 December 2015 6,397 3 View

How can I reduce basal expression with glucose in BL21DE3?

I am trying to use glucose to suppress the level of uninduced expression in my BL21DE3 by using 1% glucose but see no difference between uninduced versus induced profiles.Is it necessary to...

29 January 2015 1,750 14 View

Does anyone have experience with pRSETA ligation with a 624 bp insert and getting positive result when doing PCR but no release of insert?

My reasoning is that the EcoRI site is lost after ligation Another issue is that the Self colony is also showing positive in PCR after ligation Please see gel pictures and suggest me a reason for this

21 December 2013 10,003 5 View