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Questions related to Bioscience
I'm culturing CHO cell using media of SAFC bioscience. When culturing at 20 ℃ , there is no pellet. However, when culturing at 37 ℃, the brown pellet come out after centrifuge. Is there any...
12 June 2020 3,773 3 View
Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...
04 January 2020 3,745 4 View
01 January 2020 4,003 4 View
I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...
23 September 2019 3,089 3 View
As internet usage increase web application development become a challenge, because it is mostly customer requirement that web application must be usable on both desktop computer and...
06 August 2019 3,419 3 View
I want to use PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector (System Bioscience catalog number PB510B-1). I am trying to get its full sequence. Does anybody have this full...
06 June 2019 8,153 1 View
Please distinguised colleagues, Anyone aware of post doc opening in canada for those in Bioscience?
14 May 2019 466 2 View
Dear all, I'm performing a Miller von Gieson staining for elastin with a kit from Biosciences on porcine skeletal muscle. As a control for this kit I perform the staining on sections of arteries...
05 May 2019 2,871 1 View
05 May 2019 7,342 2 View
01 May 2019 4,220 1 View
I am interested in your experiences and opinions on "Twist Bioscience". I want to order synthesized genes and they seem to have very competetive prices. Unfortunately they don't have any...
23 April 2019 8,209 2 View
1. Unang Supratman et al. (2001). Antitumor promoting activity of Bufadienolides from Kalanchoe pinnata and K. daigremontiana tubflora. Bioscience Biotechnology and Biochemistry 65(4):947-9 2....
26 March 2019 3,356 3 View
03 March 2019 6,072 4 View
Will there always be a necessity to patent in this area of bioscience?
18 January 2019 4,525 2 View
I want to concentrate the lentiviral particles from the harvested supernatants after HEK-293T co-transfection. Since there is no ultracentrifuge available I was looking into the different kits...
07 January 2019 7,619 10 View
01 January 2019 7,008 2 View
01 January 2019 4,414 11 View
Bacteria reduction is generally represented in log scale varying from 1 log reduction onward upward 9 log. However, this only represent 99.99999... reduction. So how to represent 100 % bacteria...
26 November 2018 1,102 4 View
the cells were treated with 25uM of camptothecin for 3h then washed and fixed with 4% formaldehyde for 10min. Permeabilization was done with 90% methanol for 5 min @ RT. Blocking was done for an...
21 November 2018 7,548 2 View
11 November 2018 9,952 3 View
I was testing the binding of my protein to different lipids on a membrane lipid strip (Echelon Biosciences Inc). In contrast to my predecessor I did not see any binding at a specific lipid...
11 November 2018 4,692 0 View
I tried to monitored CD107a labelling in mouse NK cells after infection (compared to uninfected) but I'm unhappy with the plot I got. CD107a Ab clone 1D4B rat IgG2a,k from BD Bioscience cell are...
10 October 2018 4,826 2 View
I'm trying to detect the expression of cleaved caspase3 in mouse brain by western blot. Basically, my protocol is as followings: 1, lysis buffer: RIPA (ThermoFisher Science) 2, 50ug protein was...
08 August 2018 4,760 0 View
Hi everyone, I'm a new PhD student working on DC metabolism and gonna use seahorse machine for the first time. I'm the first in my lab to use the machine so don't have much guidance. I'm wondering...
08 August 2018 9,863 2 View