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Hello, So I have sonicated some bacteria culture and gathered protein cell debris mix. I want to perform SDS Page and some chromotography procedures a few months-years later from these very...
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Hello I tried checking Protbase Expasy etc but I am a noob in proteins :) So I am looking for EGF protein NOT EGFR. As far as I see its a single pass type 1 membrane protein. Since EGF binds to...
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Hello, I have been reading about inclusion bodies and refolding steps. My protein is found in inclusion bodies in Ecoli. I will perform refolding steps but I was wondering if you can add some...
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Hello I am planning to perform SDS Gel electrophoresis and my protein is about 6kb. I was checking on BioRad and saw many kinds of precast gels. 1) How should i decide whether to use Tris Hcl or...
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Hello I am new to cloning and repating an experiment. I read on promega that i need to dephosphorylate vectors when i am subcloning. Should i take the gene away from 1st vector and put the insert...
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