ec Sener

5 Questions 18 Answers 0 Followers

Questions related from ec Sener

Any tips on storing protein at -80?

Hello, So I have sonicated some bacteria culture and gathered protein cell debris mix. I want to perform SDS Page and some chromotography procedures a few months-years later from these very...

29 June 2016 1,080 10 View

Ever worked with EGF? Is EGF an introcellular or extracellular protein?

Hello I tried checking Protbase Expasy etc but I am a noob in proteins :) So I am looking for EGF protein NOT EGFR. As far as I see its a single pass type 1 membrane protein. Since EGF binds to...

27 June 2016 8,854 1 View

Do you have any suggestions to avoid inclusion bodies and promote correct protein folding?

Hello, I have been reading about inclusion bodies and refolding steps. My protein is found in inclusion bodies in Ecoli. I will perform refolding steps but I was wondering if you can add some...

16 May 2016 8,537 7 View

Ever used SDS Page Precast Gels (mini protean vs criterion) ?

Hello I am planning to perform SDS Gel electrophoresis and my protein is about 6kb. I was checking on BioRad and saw many kinds of precast gels. 1) How should i decide whether to use Tris Hcl or...

10 May 2016 6,653 3 View

How/when should i do vector dephosphorylation in subcloning?

Hello I am new to cloning and repating an experiment. I read on promega that i need to dephosphorylate vectors when i am subcloning. Should i take the gene away from 1st vector and put the insert...

06 May 2016 9,075 9 View