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Questions related from Cate Louise Williams
Hello all, I'm looking for experiences and opinions on the RiboMinus Bacteria 2.0 kit (Invitrogen) and the MICROBExpress Bacteria mRNA enrichment kit (Ambion). What have your results been like?...
06 April 2023 9,957 0 View
I have potential dimerisation in all of my primers ranging from a 3 bp overlap to 6 bps.... The PCR is not working and I have what I believe to be evidence of dimerisation on the gel, but I'm not...
15 September 2017 1,487 4 View
I am using spirit blue agar to screen for lipases in my phage-based metagenomic library. This is proving problematic and I would like to go back to the basics, however, nowhere online can I find...
26 July 2016 1,238 0 View
I accidentally used formamide instead of dimethylformamide to make up my X-gal, it resuspended fine, but can I still use it in my blue/white colour colony selection?I might add that I don't have...
27 November 2015 2,942 2 View
So, I synthesised my first strand of cDNA, and used the above kit to clean it up. However I am now concerned that the kit is for dsDNA only, and that my ssDNA may have passed through the column...
17 July 2015 5,863 3 View
I need to grow my E.coli host strain to late log phase in LB broth, however I cannot find an approximate time that the cells would hit late log phase. I appreciate that it is approximate, and I...
06 July 2015 6,681 4 View
Hi all,I have run a bacterial PCR on my polyA tailed RNA, to check for DNA contamination. The RNA is from the rumen protozoa. I have 2 bands, indicative of RNA (I think) however the upper band...
20 May 2015 8,360 1 View
