I'd like to know if plasma circulating DNA of host can be quantified instead of proviral DNA of an RNA virus, and what are the implications of doing the former instead of the latter. Thank you
I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...
03 April 2019 9,758 4 View
I did a meta-analysis using STATA and the forest plot was auto-generated after the results were calculated. However, the graph has only the study names and years included on the left side. What...
06 July 2018 9,107 0 View
A forest plot was generated from my meta-analysis, containing the study name and year. However, I would like the graph to be sorted or presented according to regions; this means that I need the...
06 July 2018 8,356 3 View
I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...
05 June 2018 4,681 3 View
Please I would like to know , what are the criteria for exclusion mapping in familial cases?
01 February 2018 665 2 View
I want to run ELISA on samples that have been stored for more than a year. Will the result be affected by storage time? If so, what troubleshooting steps do I need to alleviate this? This is...
01 February 2018 787 0 View
How do I isolate plasma circulating DNA of host to HTLV?
08 September 2017 7,421 2 View
I'd like to know the non-immune genes responsible for host susceptibility to HTLV.
08 September 2017 4,450 1 View
Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...
06 July 2016 2,661 6 View
I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...
06 July 2016 3,731 5 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View