I'd like to know if plasma circulating DNA of host can be quantified instead of proviral DNA of an RNA virus, and what are the implications of doing the former instead of the latter. Thank you
I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...
03 April 2019 9,690 4 View
I did a meta-analysis using STATA and the forest plot was auto-generated after the results were calculated. However, the graph has only the study names and years included on the left side. What...
06 July 2018 9,030 0 View
A forest plot was generated from my meta-analysis, containing the study name and year. However, I would like the graph to be sorted or presented according to regions; this means that I need the...
06 July 2018 8,252 3 View
I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...
05 June 2018 4,602 3 View
Please I would like to know , what are the criteria for exclusion mapping in familial cases?
01 February 2018 562 2 View
I want to run ELISA on samples that have been stored for more than a year. Will the result be affected by storage time? If so, what troubleshooting steps do I need to alleviate this? This is...
01 February 2018 706 0 View
I'd like to know the non-immune genes responsible for host susceptibility to HTLV.
08 September 2017 4,385 1 View
How do I isolate plasma circulating DNA of host to HTLV?
08 September 2017 7,366 2 View
I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...
06 July 2016 3,661 5 View
Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...
06 July 2016 2,598 6 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...
23 February 2021 7,326 5 View