I need help with exclusion Mapping in genetics studies?

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How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

03 April 2019 9,690 4 View

What STATA command can I use to include subgroups in my Forest plot?

I did a meta-analysis using STATA and the forest plot was auto-generated after the results were calculated. However, the graph has only the study names and years included on the left side. What...

06 July 2018 9,030 0 View

How do I group/stratify my Forest plot in STATA?

A forest plot was generated from my meta-analysis, containing the study name and year. However, I would like the graph to be sorted or presented according to regions; this means that I need the...

06 July 2018 8,252 3 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

05 June 2018 4,602 3 View

What troubleshooting steps can I use for 2 step incubation sandwich ELISA?

I want to run ELISA on samples that have been stored for more than a year. Will the result be affected by storage time? If so, what troubleshooting steps do I need to alleviate this? This is...

01 February 2018 706 0 View

Can host ccfDNA be quantified instead of viral gene?

I'd like to know if plasma circulating DNA of host can be quantified instead of proviral DNA of an RNA virus, and what are the implications of doing the former instead of the latter. Thank you

09 October 2017 3,572 0 View

What are the non-immune host genes that determine suceptibility/resistance to HTLV I/II?

I'd like to know the non-immune genes responsible for host susceptibility to HTLV.

08 September 2017 4,385 1 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

08 September 2017 7,366 2 View

What is the ideal tube for storing serum samples at -20 or -80 degrees (after centrifugation and separation from whole blood) as the case may be?

I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...

06 July 2016 3,661 5 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

06 July 2016 2,598 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View