How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

03 April 2019 9,690 4 View

What STATA command can I use to include subgroups in my Forest plot?

I did a meta-analysis using STATA and the forest plot was auto-generated after the results were calculated. However, the graph has only the study names and years included on the left side. What...

06 July 2018 9,030 0 View

How do I group/stratify my Forest plot in STATA?

A forest plot was generated from my meta-analysis, containing the study name and year. However, I would like the graph to be sorted or presented according to regions; this means that I need the...

06 July 2018 8,252 3 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

05 June 2018 4,602 3 View

I need help with exclusion Mapping in genetics studies?

Please I would like to know , what are the criteria for exclusion mapping in familial cases?

01 February 2018 562 2 View

Can host ccfDNA be quantified instead of viral gene?

I'd like to know if plasma circulating DNA of host can be quantified instead of proviral DNA of an RNA virus, and what are the implications of doing the former instead of the latter. Thank you

09 October 2017 3,572 0 View

What are the non-immune host genes that determine suceptibility/resistance to HTLV I/II?

I'd like to know the non-immune genes responsible for host susceptibility to HTLV.

08 September 2017 4,385 1 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

08 September 2017 7,366 2 View

What is the ideal tube for storing serum samples at -20 or -80 degrees (after centrifugation and separation from whole blood) as the case may be?

I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...

06 July 2016 3,661 5 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

06 July 2016 2,598 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View