N.C.J Anyanwu

11 Questions 8 Answers 0 Followers

Questions related from N.C.J Anyanwu

How do I preserve stool samples until the required sample size needed for further analysis is obtained?

I'm currently collecting stool samples for Rotavirus ELISA assay. Please how do I preserve the stool samples until number of samples needed are complete and how long can I preserve these samples...

04 April 2019 9,741 4 View

How do I group/stratify my Forest plot in STATA?

A forest plot was generated from my meta-analysis, containing the study name and year. However, I would like the graph to be sorted or presented according to regions; this means that I need the...

07 July 2018 8,321 3 View

What STATA command can I use to include subgroups in my Forest plot?

I did a meta-analysis using STATA and the forest plot was auto-generated after the results were calculated. However, the graph has only the study names and years included on the left side. What...

07 July 2018 9,083 0 View

Could anyone please help me with step-by-step guide on how to run my meta-analysis on STATA 14.2?

I want to do a meta-analysis of previous prevalence studies for 2 subspecies of an organism in a particular location and I need to calculate/derive these: 1. Confidence Interval for individual...

06 June 2018 4,659 3 View

What troubleshooting steps can I use for 2 step incubation sandwich ELISA?

I want to run ELISA on samples that have been stored for more than a year. Will the result be affected by storage time? If so, what troubleshooting steps do I need to alleviate this? This is...

02 February 2018 748 0 View

I need help with exclusion Mapping in genetics studies?

Please I would like to know , what are the criteria for exclusion mapping in familial cases?

02 February 2018 636 2 View

Can host ccfDNA be quantified instead of viral gene?

I'd like to know if plasma circulating DNA of host can be quantified instead of proviral DNA of an RNA virus, and what are the implications of doing the former instead of the latter. Thank you

10 October 2017 3,613 0 View

What's the best protocol for isolating plasma circulating host DNA to HTLV?

How do I isolate plasma circulating DNA of host to HTLV?

09 September 2017 7,405 2 View

What are the non-immune host genes that determine suceptibility/resistance to HTLV I/II?

I'd like to know the non-immune genes responsible for host susceptibility to HTLV.

09 September 2017 4,428 1 View

What is the effect of non-vacuum plain tube on blood serum as compared to vacutainer plain tubes?

Some hospitals, due to cost, (especially in the under-developed and developing countries) use non-vacuum plain tubes for serum where methodology stipulates the use of vacutainer plain tubes. Can I...

07 July 2016 2,641 6 View

What is the ideal tube for storing serum samples at -20 or -80 degrees (after centrifugation and separation from whole blood) as the case may be?

I am presently sampling serum from patients for further processing in 6 months or more. I would like to ascertain the ideal tube for storage of these samples to avoid contamination, leakage,...

07 July 2016 3,703 5 View