Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

31 December 2015 6,300 4 View

Is it possible for cells not to metabolize MTT labelling reagent?

Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...

07 August 2015 6,503 7 View

Could anyone suggest techniques / assays for testing for 2 of the Hallmarks of cancer?

Could anyone suggest techniques for testing the following 2 Hallmarks of Cancer: 1. Resisting Cell Death & 2. Activating Invasion and metastasis? Any literature on this would be appreciated as...

10 November 2014 5,684 14 View

Any good reviews on the Hallmarks of Cancer?

Good day all. Does anybody know of any good (recent) review papers on 1. Resisting cell death in cancer & 2.Activating invasion and metastasis? Other than the Hanahan and Weinberg paper. Thanks

10 November 2014 915 1 View

Is anyone able to share melanoma disease progression literature?

I am doing a study on the protein expression profile of melanoma progression (RGP-->VGP-->metastatic melanoma) and looking out for any literature on the molecular changes (at DNA &...

06 July 2014 1,627 2 View

What is the lowest amount of RNA that one can load on a gel in order to check RNA integrity?

I have very little RNA samples and therefore it is quite precious. I need to check RNA integrity and would like to get opinions on what the lowest advisable amount of RNA is when loading on a gel....

02 March 2014 8,564 6 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View