Alexis Neumann

7 Questions 9 Answers 0 Followers

Questions related from Alexis Neumann

Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

Good day all, I am currently trying to familiarize myself with the culturing and maintenance of HL60 & JURKAT leukemia suspension cultures. What density should these cells be cultured at, how...

01 January 2016 6,333 4 View

Can DMSO concentrations below 0.1% affect MCF7 cells?

Hi all, So I am treating MCF7 cells with a drug and I have a vehicle (DMSO) control. The DMSO in the control is at 0.022% which is below the limit of 0.1% but I am seeing stress responses in my...

08 August 2015 7,223 2 View

Is it possible for cells not to metabolize MTT labelling reagent?

Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...

08 August 2015 6,566 7 View

Any good reviews on the Hallmarks of Cancer?

Good day all. Does anybody know of any good (recent) review papers on 1. Resisting cell death in cancer & 2.Activating invasion and metastasis? Other than the Hanahan and Weinberg paper. Thanks

11 November 2014 956 1 View

Could anyone suggest techniques / assays for testing for 2 of the Hallmarks of cancer?

Could anyone suggest techniques for testing the following 2 Hallmarks of Cancer: 1. Resisting Cell Death & 2. Activating Invasion and metastasis? Any literature on this would be appreciated as...

11 November 2014 5,717 14 View

Is anyone able to share melanoma disease progression literature?

I am doing a study on the protein expression profile of melanoma progression (RGP-->VGP-->metastatic melanoma) and looking out for any literature on the molecular changes (at DNA &...

07 July 2014 1,659 2 View

What is the lowest amount of RNA that one can load on a gel in order to check RNA integrity?

I have very little RNA samples and therefore it is quite precious. I need to check RNA integrity and would like to get opinions on what the lowest advisable amount of RNA is when loading on a gel....

03 March 2014 8,602 6 View