I have very little RNA samples and therefore it is quite precious. I need to check RNA integrity and would like to get opinions on what the lowest advisable amount of RNA is when loading on a gel. This could be either a normal agarose or formaldehyde gel. Thanks a mil.
Do you have access to an Agilent Bioanlyzer? They have a pico chip that performs pretty well. It is good for approx 0.8-8.0ng/uL, using ~1uL of sample.
Do you have access to an Agilent Bioanlyzer? They have a pico chip that performs pretty well. It is good for approx 0.8-8.0ng/uL, using ~1uL of sample.
The usual molecular cloning manual statement about this is 10 ug RNA/lane.
Since RNA is a poor intercalator of dyes like ethidium bromide, a lot more has to be loaded on gels to visualize it.
Rohan and Roberta above have provided your absolute best option.
If you can go for a Northern Blot, you can detect less than 0.1 fmol of RNAs.
Best.
How long is your RNA? If more than 200-300 nt then you can see even 50 ng on formaldehyde agarose gel with ethidium bromide.
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