Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

31 December 2015 6,300 4 View

Can DMSO concentrations below 0.1% affect MCF7 cells?

Hi all, So I am treating MCF7 cells with a drug and I have a vehicle (DMSO) control. The DMSO in the control is at 0.022% which is below the limit of 0.1% but I am seeing stress responses in my...

07 August 2015 7,189 2 View

Is it possible for cells not to metabolize MTT labelling reagent?

Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...

07 August 2015 6,503 7 View

Could anyone suggest techniques / assays for testing for 2 of the Hallmarks of cancer?

Could anyone suggest techniques for testing the following 2 Hallmarks of Cancer: 1. Resisting Cell Death & 2. Activating Invasion and metastasis? Any literature on this would be appreciated as...

10 November 2014 5,684 14 View

Any good reviews on the Hallmarks of Cancer?

Good day all. Does anybody know of any good (recent) review papers on 1. Resisting cell death in cancer & 2.Activating invasion and metastasis? Other than the Hanahan and Weinberg paper. Thanks

10 November 2014 915 1 View

What is the lowest amount of RNA that one can load on a gel in order to check RNA integrity?

I have very little RNA samples and therefore it is quite precious. I need to check RNA integrity and would like to get opinions on what the lowest advisable amount of RNA is when loading on a gel....

02 March 2014 8,564 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View