Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

31 December 2015 6,300 4 View

Can DMSO concentrations below 0.1% affect MCF7 cells?

Hi all, So I am treating MCF7 cells with a drug and I have a vehicle (DMSO) control. The DMSO in the control is at 0.022% which is below the limit of 0.1% but I am seeing stress responses in my...

07 August 2015 7,189 2 View

Is it possible for cells not to metabolize MTT labelling reagent?

Hi all. I have performed an MTT cell viability assay and the vehicle controls do not seem to be metabolizing the MTT reagent. I am basing this on the fact that I definitely see live adherent cells...

07 August 2015 6,503 7 View

Could anyone suggest techniques / assays for testing for 2 of the Hallmarks of cancer?

Could anyone suggest techniques for testing the following 2 Hallmarks of Cancer: 1. Resisting Cell Death & 2. Activating Invasion and metastasis? Any literature on this would be appreciated as...

10 November 2014 5,684 14 View

Is anyone able to share melanoma disease progression literature?

I am doing a study on the protein expression profile of melanoma progression (RGP-->VGP-->metastatic melanoma) and looking out for any literature on the molecular changes (at DNA &...

06 July 2014 1,627 2 View

What is the lowest amount of RNA that one can load on a gel in order to check RNA integrity?

I have very little RNA samples and therefore it is quite precious. I need to check RNA integrity and would like to get opinions on what the lowest advisable amount of RNA is when loading on a gel....

02 March 2014 8,564 6 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View