Is it possible that cells, specially those with purinergic system, incorporate Ethidium Homodimer-1 (EthD-1) without dying? For example, in the Live & Dead kit, can EthD-1 labelled cells not be dead after all?
Could Britain's past Colonial and Slave legacy. Be in anyway still having an adverse effect on the mental health and wellbeing of black people. From Education Socially and incarceration.
25 June 2024 4,785 1 View
Can someone please clarify the correct way to calculate LogID50 using the Reed and Muench method. Using the example values below: 10^-1 100% positive 10^-2 100% positive 10^-3 100% positive 10^-4...
01 May 2024 4,113 0 View
Are there new projects and studies considering recursive approaches, in energy transition and resources, being empirically tested with regression analysis and models?
14 April 2024 7,024 4 View
What are the key findings and implications of the study that aimed to optimize supplier control in the aviation industry, and how might these findings be applied to improve supplier control in...
06 November 2023 2,910 1 View
How is tristyrylphenol is produced and which catalyst is used industrially?
20 October 2023 5,090 2 View
Journal on culture?
07 March 2023 4,502 3 View
Watching movies and interacting with visual media are engaging ways to help students learn. So, I would like to stream a movie, TV show, or documentary as part of a lesson for undergrad students...
10 January 2023 1,804 4 View
Eco-training in Pestalozzi: without love, without the acceptance of the other at our side, there is no socialization, and without socialization there is no humanity. Understanding the phenomenon...
01 June 2022 3,352 2 View
This question is directed to professors who work in design education. I want to get an overview of ways to assess skills in students.
20 January 2022 4,402 4 View
Dear all, We are currently conducting a meta-analysis on the acoustic adaptation hypothesis across animals. We aim to test whether habitat structure predicts animal vocalisations (within and...
18 October 2021 1,654 0 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View
I'm performing interactions between DNA as the macromolecule and Ethidium Bromide as the ligand. I'm looking to make the entire DNA molecule flexible in order to perform blind docking. However, I...
10 June 2024 6,270 1 View
Greetings, dear colleagues! Do you know any technique utilizing some ~ordinary molbiol lab reagents (not the commertial kits for isolated mitochondria staining) that will allow isolated...
07 June 2024 5,909 0 View