Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

Has anybody used computational linguistics-based, AI-assisted analytics for their research like the covid-19 study below?

16 November 2020 8,316 3 View

Is it possible to convert WRF output data (netcdf) to grib?

I'd like to use WRF output data to force a land surface model (HRLDAS). HRLDAS requires grib2 forcing data and I have so far been unable to convert from netcdf to grib. There are a number of tools...

17 March 2020 8,962 6 View

Is it possible to find distance of objects in an image using AI?

I am working on a project for my school, the projects are unique and based on real world problems so there really could not be a solution. So here it goes. I have a bunch of photos of fields,...

03 March 2020 6,951 5 View

Are you a motivational intervieiwng trainer/practitioner/researhcer based in Western Australia?

If you are focussed on (or just interested in MI) and based in WA please get in touch- I am interested in potential collaborations for practice research and training, kind regardsTrevor.

05 February 2020 6,084 1 View

Germany on chain: National Blockchain Strategy Released - Thoughts?

Article Link: https://medium.com/@philippsandner/germany-national-blockchain-strategy-released-1420e9d066f7

18 September 2019 5,237 4 View

Have your heard of Deloitte's new term "The Symphonic Enterprise"?

"In the symphonic enterprise, the old lines become blurred, thus creating a diagonal view that illuminates new business opportunities and creative ways of solving the most complex problems via the...

19 August 2018 3,894 3 View

No title

15 November 2017 8,055 2 View

What is the best way to preserve (fish) tissues for PacBio sequencing?

04 March 2017 6,232 3 View

Moving from cyclic voltammetry to bulk electrolysis for a mediated enzyme reaction. How do I work out what mediator concentration I should use?

23 October 2014 1,228 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View