Hércules Rezende Freitas

3 Questions 19 Answers 0 Followers

Questions related from Hércules Rezende Freitas

How's viability for frozen (-80ºC) rat brain tissue in cell culture?

I'm planning to use frozen (-80ºC) rat brain tissue for cell culture purposes, will I obtain good viability? Can I culture these cells using supplemented DMEM-F12 (with FBS and B27)?

16 November 2017 8,133 2 View

Can cells incorporate Ethidium Homodimer-1 without dying?

Is it possible that cells, specially those with purinergic system, incorporate Ethidium Homodimer-1 (EthD-1) without dying? For example, in the Live & Dead kit, can EthD-1 labelled cells not...

13 November 2017 8,324 0 View

Mixed rat cortical cultures using supplemented DMEM-F12?

Dear colleagues, Can I culture rat cortical cells on supplemented DMEM-F12? Is there a paper comparing medium culture conditions? Sincerely, Hércules Freitas

09 November 2017 570 1 View