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Questions related from Hércules Rezende Freitas
Original papers and a little about your research, I'm collaborating with a group and need to read some of the literature, maybe cite it too.
17 November 2017 4,951 0 View
I'm planning to use frozen (-80ºC) rat brain tissue for cell culture purposes, will I obtain good viability? Can I culture these cells using supplemented DMEM-F12 (with FBS and B27)?
16 November 2017 8,219 2 View
Is it possible that cells, specially those with purinergic system, incorporate Ethidium Homodimer-1 (EthD-1) without dying? For example, in the Live & Dead kit, can EthD-1 labelled cells not...
13 November 2017 8,373 0 View
Dear colleagues, Can I culture rat cortical cells on supplemented DMEM-F12? Is there a paper comparing medium culture conditions? Sincerely, Hércules Freitas
09 November 2017 618 1 View