Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How to generate .dlg files on PyRx?

Hi. I'm using the PyRx version 0.8 and getting the error message: WARNING: Filename mismatch: "2DFN_ADP_CL.maps.fld" :: "..\2DFN_ADP_CL.maps.fld" C:\Program Files (x86)\The Scripps Research...

17 January 2021 843 3 View

Confirming what statistics I need to run for a pilot study in social sciences?

I have altered two instruments and collected a sample of n=60. 1. For the first instrument, I changed the wording of the items to refer to life instead of work. I believe checking the cronbach...

13 March 2020 7,391 3 View

Recovery rate for PD-10 desalting column: Any idea?

Hi all, I have high levels of protein loss on PD-10 desalting column (gavity flow desalting column) when trying to buffer exchange. According to GE, the recovery rate should be higher that 70%,...

03 October 2019 8,483 3 View

Why did I get RMSD plot with 10ns if the MD was 100ns trajectory?

I am new in gromacs and I'm experiencing some difficulties in the analysis of my results. I calculated the RMSD of backbone and ligand from the MD with a trajectory of 100ns (nsteps = 50000000),...

04 September 2019 3,669 6 View

Reversed Flow in Spillway Simulation in Fluent?

Hi I'm trying to simulate the flow of water over an ogee spillway in Fluent. My problem is that I'm getting reversed flow on my pressure outlets. I am aware that it might not affect significantly...

05 December 2018 6,108 3 View

How can I quantify exosomes protein from cell culture media?

I've isolated exosomes from 5ml of cell culture media using ExoQuick TC PLUS kit. I got a visible pellet that was resuspended in the "resuspension buffer" and then purified with the...

13 June 2018 5,514 4 View

What method is best or standard to determine Glut1 expression in T cells?

I am trying to decide the best method between flow cytometry or qPCR to be used as I compare Glut1 expression in two different T cell lines. Is there an ideal protocol? I had read articles where...

17 March 2018 4,211 3 View

How to Build a silica nanosphere with Materials Studio?

Hello, I am beginner in Materials studio software and also in Molecular dynamics and I am needing to build a silica nanosphere with different numbers of hydroxyl groups to adsorb some molecules....

27 December 2017 5,577 2 View

No title

15 November 2017 8,055 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Which one carries more value? A research paper in a Scopus Indexed conference proceedings or the same paper in a Journal without Scopus indexing?

I am looking for the suggestion among which one to pick from the above two categories for a research work.

27 February 2021 10,025 1 View

Which indexation is better Scopus or Web of Science?

Both are very repetitive indexing agencies.

26 February 2021 9,455 4 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How to approach CFD simulation results of a flow past a cylinder?

Hi all, I'm doing a transient simulation of flow past a rectangular cylinder close to the free surface. As far as I'm concerned, the common practice is to discard the initial stages of the...

25 February 2021 1,348 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 9,648 3 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

How do I add a udf to Ansys fluent and does it need to be compiled before hand ?

I have a Define_CG_Motion udf written in c where do I add it to my Ansys fluent project and does it need to be compiled before that?

21 February 2021 9,347 3 View

Which is the best software or programming language (user friendly) for analysing data?

I have a basic knowledge on Python, R and MATLAB in analysing data. Among those, which is the best and user friendly? Which one should I learn deeply to analyse data?N:B: I want to learn data...

20 February 2021 3,950 15 View