I have to sub-clone a gene from one vector into another. However I cannot find the correct plasmid with correct MCS. What do I do?
what's your goal for subcloning? for gene expression you should select an expression vector based on your favorit host.
what do you mean by correct plasmid or correct MCS? it is better to explain more about your problem.
In metaanalysis or systematic review, forest plots are made to compare different data set as random trial. I need to learn that .help needed.
03 July 2020 9,598 4 View
27 January 2016 5,610 9 View
I need to perform QSAR and pharmacokinetic study in silico. Please guide me with any server or software which i can freely access and which is quite reliable as per results are concerned.
17 December 2015 301 12 View
Can +3 cations form tartrate complexes?
25 October 2015 3,094 3 View
Hi everyone!! While performing the MD simulations on proteins and studying their unfolding by Guanidium, How should one determine the guanidium concentration with regard to the number of molecules...
29 July 2015 4,437 5 View
A ligand showing different effect at different pH. I wanted to study the interaction of the ligand with DNA. So how will i study those at different pH environment through molecular docking...
09 October 2014 5,874 3 View
I am writing a project and need to create at least some data pool to send my first independent project. If anyone in this research family can provide me ghrelin peptide, in very small amount, I...
01 January 1970 6,075 3 View
Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1
17 August 2021 0 0 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
20 June 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help
08 May 2021 0 0 View