Can anyone help me in finding a place to do my DNA microarray analysis for my sample?

More Sathya Mylsamy's questions See All

How the cumulative percentage of overall work completion (for construction projects) scheduled for every month in real projects ?

By checking the monthly project progress report of any construction project, we can track the actual cumulative project progress in percentage. But it may differ from the planned cumulative...

10 April 2023 733 4 View

How to coat the polymer material over the natural fibre ?

Natural Fibre Coating

13 July 2021 6,495 5 View

Can we run an EFA before a SEM?

I'm conducting a study for my PhD thesis. I've got some constructs from the literature, but the items to measure the constructs were modified (some were added and some were removed). In this case,...

08 June 2020 6,165 9 View

Any one suggeste about the meta analysis for urinary bladder cancer

I am looking forward about the meta analysis for urinary bladder cancer

26 May 2017 3,438 1 View

Non linear FEA on masonry wall using Ansys APDL?

Hello everyone . I am doing a non linear FEA on Masonry. I have to analyse the plasticity behaviour of the model using Ansys APDL. I have to analyse the effects under the effect of wind load and...

21 March 2017 9,079 3 View

What is the best stimulant to differentiate naive macrophage from monocytes ?

I would like to compare the expression profile of naive macrophage(M0) with Classically Activated M1 and Alternatively activated macrophage M2. People use M-CSF (Monocyte -colony stimulating...

12 July 2015 1,884 3 View

In modifying a source term in wet steam model, how can I solve for moist air ?

Greetings everybody, I am trying to solve moist air condensation through a nozzle using ANSYS Fluent's WET STEAM model. I have written UDFs to modify the wet steam properties (UDWSPF). In the...

12 June 2015 1,541 2 View

Does anyone know how to predict SNPs in a particular gene?

I have data for which differential gene expression was found. Now I am interested in finding SNPs only in the genes which are differentially expressed (gene name is known). Could someone suggest...

26 February 2014 6,425 2 View

I am interested in finding the markers for urinary bladder cancer for diagnosis. Please help me in finding the markers via microarray.

Working plate form.

15 February 2013 7,197 1 View

Concerning membrane damage during cancer

I would like to know the possible effects on the cell mambranes due to proteins and lipids with the onset of liver cancer.I am working on a project based on the same topic.

08 January 2010 7,145 8 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Panagiotis Apostolou

What microarrays experiments do you want to do? Gene expression, SNPs? Do you interest for a specific platform? I need more details about the experiment.

Sten Even Erlandsen

Hi,

I do not want to promote any commercial bussines, but

http://www.illumina.com/services/corelabs.ilmn is a good place to start.

Overview of Core facilities around the world offering serveral different assays based on illuina technology.

Sathya Mylsamy

I need to find out the tumor markers in the graded tumors.