A forum to address questions regarding methods of protein purification. | Contact experts in Protein Purification to get answers
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Questions related to Protein Purification
I need to know liquid-liquid extraction with the right quantities of PEG-salt ratio, also the volume ratio, phase diagram.
11 August 2023 2,207 1 View
Hello everyone, I'm having some conceptual misunderstandings regarding non-reducing SDS-PAGE. In this situation, we omit reducing agents such DTT or BME from the loading buffer to preserve...
19 July 2023 3,610 2 View
The size differ little bit from one species to another, yet they have one size range. Also, the size of them in their native form so they don't lose their colour while isolation.
12 July 2023 9,418 2 View
No
12 July 2023 8,384 1 View
One Thing, Your Physics Book Many Equations Depend On Uniform Velocity And Acceleration Equation But Nothing Is Uniform All Time And Change Should Be And Must Be Come Every Where In Universe About...
02 July 2023 9,430 1 View
The size of the protein is 154KDa his-tagged. I have tried inducing with 0.5mM IPTG for 4hrs at 37 degrees. I have tried different strains BL21-De3 Gene X, shuffle T7, and artic but still no...
28 June 2023 2,417 1 View
By keeping rest of the protocol same does centrifugation speed will have any effect on protein extraction from XL1-blue colonies
27 June 2023 2,273 3 View
suppose: we have a purified protein with us; after this step what are the methods and procedures that can we use to see the protein is intact- KINDLY HELP
02 June 2023 3,516 3 View
I want to generate the electron.dat file for my lipid membrane systems and i am unable to figure it out that how i can get electron.dat file so that i can use gmx_density feature of gromacs. I...
01 June 2023 3,795 0 View
I am working on a recombinant protein using SDS-PAGE. I used Coomassie brilliant blue for visualization of the respected protein bands. The voltage was set at 150V and the gel was run for 1 hour....
29 May 2023 382 2 View
I want to extract glycosaminoglycans using pepsin enzyme, I tried Tris buffer and sodium acetate at pH 1-3, but I want to try a buffer other than these two, thank you for guiding me.
08 May 2023 1,573 5 View
suppose X is the total polarizability and x1 and x2 are individual polarizabilities. So will the final result be X=x1+x2? Please help.
09 April 2023 7,979 2 View
My protein has no protease (relevant to the E. coli system)degradation site, as I have checked on expasy. I store my protein at 4 degrees as it aggregated at -20 degrees. It is degrading over time...
04 March 2023 1,062 6 View
I am doing a histomorphological analysis of broiler chickens at 21 and 42 days of age. I want to take images using an OLYMPUS microscope (BX43) to observe the slices and take photos. In the...
25 February 2023 4,956 3 View
I cant get rid of this artefact on my EVG slides. It's not caused by drying slides as it occurs when I dont dry the slides, they are just less yellow. Connective tissues look great. Any advise...
13 January 2023 7,143 0 View
Hi, I am performing QSAR approach to design and identify new inhibitors by using an online application tool. I have completed whole steps, but unable to generate counter energy map.
08 January 2023 1,668 4 View
Does any have a method for analyzing VOCs in water? or VOCs solvent? I study VOCs degradation in water by microorganisms.
02 January 2023 8,391 2 View
I am having some trouble with the transformation protocol of electrocompetent TOP10 E. coli cells. At first I thought it could be because of the size of the plasmid (10 kb) so I electroporated...
26 December 2022 6,948 5 View
Hello everyone, I only ask for help on ResearchGate when I can't find a proper answer out there. I want to start experimenting with statins in PBMC cultures and I've been looking for a...
14 December 2022 2,596 2 View
I'm using Ni-Nta beads to purify the His-tag recombinant protein. After sonication, I haven't found any dimers upon SDS-PAGE. But after that load and the next purified eluted protein formed...
06 December 2022 9,255 4 View
am trying to purify a human DNA binding protein (normally localized in nucleus and mitochondria). I am currently optimizing the conditions I tried 2 different approaches: 1. HisTrap-> TEV -->...
06 December 2022 5,022 5 View
Hi, I need to store some larvae of G. mellonella for future RNA extractions. My idea is to pre freeze them with liquid nitrogen and then put them in a eppendorf at -80. Should I add also...
01 December 2022 1,194 3 View
Hi, colleagues. Can someone assist me on how to validate my docking protocol using EF and ROC curves? I have searched through the literature i am failing to find a step-by-step approach to...
29 November 2022 2,442 2 View
I have a couple of proteins (95-130 KDa) to make them clean. What are the best microcentrifuge tube based column filtrations you guys have tried and worked best. Any suggestion with direct product...
29 November 2022 4,338 3 View