A forum to address questions regarding methods of protein purification. | Contact experts in Protein Purification to get answers
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Questions related to Protein Purification
I am going to measure the expression of 4 GPCRs in tissue sample from mouse pancreas. How can I analyse the result as I have to compare the expression of my target receptor with other receptors in...
15 August 2025 7,864 1 View
HPLC,RI-detector
14 August 2025 5,644 3 View
i am working on the nutrition value of food and read the paper (Author Correction: Levelling foods for priority micronutrien... ). In which the authors used the priority micronutrient value. I am...
22 March 2025 3,586 0 View
I am looking for a good commercially available positive control for CDC assay or is there a way to achieve 100% complement mediated killing of peripheral blood mononuclear cells or myeloma cell...
05 December 2024 8,752 0 View
How Medications Can Affect Dental Implant Treatment
16 September 2024 2,691 3 View
MC3T3-E1 (Mouse osteoblast) is cultured using Alpha-MEM, 10% FBS, 1% P/S. I'm looking at reactions to drugs, and black dots like that only appear on certain drugs. Seeing that it is reoccurring...
12 September 2024 9,429 0 View
Hello, I am conducting an experiment to induce osteoblast differentiation using MC3T3-E1 cells. Cells are treated with differentiation reagents for 7 days. After treating the cells with the...
03 September 2024 652 0 View
The Plasmodium DNA sequence will be provided if needed. Alternatively, the mapping instrument that can be able to predict potential epitopes within the DNA be provided for me to work with.
06 April 2024 7,022 0 View
In India it is not avilable on MTCC. NCMR culture is not working.
14 March 2024 6,722 0 View
I would like to confirm PCR Results, so i thought that "knock out" of gene of interest will work. Who support this? If someone has different aspect would you please share?
18 January 2024 1,493 2 View
Hello, I am working on a protein that belongs to NUDIX family hydrolases and therefore requires Mg ion as a cofactor. But the protein that I have downloaded from PDB is an apoprotein. I want to...
12 January 2024 8,169 2 View
Hi, I have Michaelis-Menten curves for 2 conditions (control VS disease) to look at the difference in activity of my enzyme of interest. I'd like to see the best way to quantify the difference in...
12 January 2024 6,523 2 View
This is a hint from the NCBI database: This controls whether the primer should span an exon junction on your mRNA template. The option "Primer must span an exon-exon junction" will direct the...
11 January 2024 5,914 0 View
Well, as you know, the stem-loop primer has a long chain, increasing the likelihood of undesirable secondary structure formation.
05 January 2024 3,151 0 View
Please provide the article's link. Thanks!
03 January 2024 8,257 0 View
Please also provide the article's link. Thanks!
03 January 2024 4,809 0 View
Hello, so, i just did a protein denaturation at 45ºC for 45 minutes in a thermal cycler for an electrophoresis for a posterior western blot. The problem is that i fu*** up the gels when i was...
07 December 2023 8,207 5 View
Also, is there a way to convert SMILES into IUPAC or common name of the compound?
01 December 2023 351 2 View
What are good resources for an undergraduate student to start getting familiar with bioinformatics and, if possible, get some practical experience? Any favorite websites, blogs, videos, etc? Thanks!
30 November 2023 1,922 6 View
I am working with HepG2 cells to observe effects of Simvastatin treatment on the cells. We are aiming to identify a dose response associated with levels of ATP, metabolic activity, and CoQ10...
30 November 2023 6,113 0 View
I'm gonna do the E. coli cell-free experiment in 384-well plate. But I have trouble with plate choice. E. coli cell-free experiment doesn't need a sterile enviroment, but I think plate should be...
13 November 2023 2,031 0 View
Dear respected All, I am planning to do MS analysis of glioma cell-derived exosomes. Sample preparation from cell line for MS analysis is well established but from exosomes, it would be different....
20 October 2023 5,254 3 View
Hello everyone! I want to know why the zeta potential of cholesterol is negative.
14 October 2023 3,236 2 View
Hi all, I have silica coated QDs using the standard microemulsion method (in cyclohexane with Igepal +TEOS, followed by repeated washing by centrifuge in ethanol). However my particles are often...
26 September 2023 1,848 2 View