Panagiotis Apostolou

5 Questions 43 Answers 0 Followers

Questions related from Panagiotis Apostolou

How to remove the aminoacids after the His-tag and before my protein?

We'd like to produce recombinant proteins using a vector with His-tag in N-terminus. Our gene is inserted after 10-15bp after the His-tag. At the final step of purification and cleavage, how can...

14 January 2016 3,047 8 View

Does anybody know any cell line resistant to alkaloids of vinca and specially on vincristine?

I'd like to study human resistance to alkaloids of the vinca cell line.

28 August 2014 7,775 3 View

Does anybody use an array image analysis software?

We would like to analyze the data from antibody array after scanning.

19 December 2013 3,459 1 View

Method for tissue explant.

I'd like to create a cell line from a tissue. Until now, I remove with trypsin the cells from the tissue and culture them. Also I keep the tissue pieces in petri dishes. In the culture I use RPMI...

14 March 2013 3,691 5 View

How can I create a resistant cell line?

I'm trying to create a cisplatin resistant cancer cell line, HCT-116. Does anyone know the optimal concentration that I must add to the culture? I started with 1μg/ml of CDDP. I noticed that the...

06 March 2013 919 30 View