10 Questions 52 Answers 0 Followers
Questions related from Panagiotis Apostolou
We'd like to produce recombinant proteins using a vector with His-tag in N-terminus. Our gene is inserted after 10-15bp after the His-tag. At the final step of purification and cleavage, how can...
14 January 2016 3,059 8 View
I've submitted a research article that deals with new biomarkers, and is based on samples received from cancer patients. The patients didn't give the blood for this project, however they consented...
31 October 2014 9,384 14 View
There are many theories trying to explain ways to create a stable transfection in mammalian cells, by selection. Does it work? Is there any other method?
29 August 2014 4,765 2 View
I'd like to study human resistance to alkaloids of the vinca cell line.
28 August 2014 7,787 3 View
I performed the ligation protocol with Promega T4 DNA ligase and I observed the ligation was ok. After that, I tried to repeat the experiments. However, in all cases (more than 3 times), there are...
24 July 2014 8,924 5 View
I'd like to perform some experiments by applying metronomic chemotherapy with 5FU and Oxaliplatin in colorectal cancer cell lines. Has anyone used a particular concentration for this application?
31 March 2014 4,590 2 View
We would like to analyze the data from antibody array after scanning.
19 December 2013 3,468 1 View
I'd like to create a cell line from a tissue. Until now, I remove with trypsin the cells from the tissue and culture them. Also I keep the tissue pieces in petri dishes. In the culture I use RPMI...
14 March 2013 3,700 5 View
I'm trying to create a cisplatin resistant cancer cell line, HCT-116. Does anyone know the optimal concentration that I must add to the culture? I started with 1μg/ml of CDDP. I noticed that the...
06 March 2013 929 30 View
With TRIZOL method the RNA is degraded and we observed high absorbance in 230 (>2,5). The ratio is good enough (1.8-2.1).
23 January 2013 986 5 View
