Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

I'm just learning the ePrime 3.0 software and have created a test. How can I put this test on a tablet for subjects to use for data collection?

This is probably a really basic question, but I can't figure it out. I've loaded the test on a USB and transferred to the tablet, but am unable to get it to run. Any help would be wonderful. Thank...

23 December 2020 5,202 3 View

How to get citations on RG to show up on Google Scholar and vice versa?

After connecting both platforms, there remains unique coverage; just this week I discovered a new citation of mine on Google Scholar, but it's not being recognized by RG?

30 October 2020 4,311 3 View

How do I calculate transition probabilities for a sequence of syllables in a set of bird songs using R?

I would like to automatically generate a Markov chain analysis for my set of bird songs that I have assigned syllable categories to using R. I have around 70 songs (each from an individual bird)...

13 October 2020 4,833 2 View

Someone knows of a DNA purification protocol from polysaccharides (for brown seaweed extracts)?

Hi, I have this "bad" DNA extract of a Fucus spp. and I want to purifying this because the PCR analysis that I need don't show results. I alredy try to extract twice the DNA both with the Genomic...

06 October 2020 1,500 1 View

Inconsistent protein expression for different maxipreps?

I have been having large variability in protein expression from transfections of mammalian cells with DNA from different maxipreps, even though the plasmid is the same. I have been using the...

02 October 2020 2,924 3 View

How to prepare a vial of Sigma Adjuvant System (Ribi) when needing multiple immunogens/at different immunisation points?

https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole...

02 September 2020 7,451 2 View

Who is investigating artificial intelligence / machine learning for leadership augmentation and resource distribution?

I've been searching various A.I. projects and have found very little on the role A.I. can play in leadership and government. If we imagine a leader who cares about their constituency and does not...

09 August 2020 1,213 2 View

How do I assess when to use "match between runs" for LFQ analysis on Maxquant?

Hi Proteomics enthusiasts, I am currently analyzing DNA pulldown data (treated vs non-treated) using Label-free quantification on Maxquant. I am trying to assess if a protein is present in one...

04 August 2020 1,090 6 View

Could you recommend a scale that measures group intelligence or the predisposition to work in a group?

For my thesis project, based on the work of Reeves and Nass reported in the book "The Media Equation", I would need a scale that goes to measure group intelligence or the predisposition to work in...

25 July 2020 1,294 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View