There are black dots that seem like contamination in my corneal endothelial cell cultures. Some of the cells appear unhealthy and are enlarged. The black dots are more prevalent around these enlarged (possibly dying or apoptotic) cells. Although they can be washed off, they reappear a few days after changing the media. They are not growing dominantly, and the media color remains normal. Only a small portion of the cells are affected. What could these contaminants be, and how can I get rid of them? Please see the images below and share your thoughts. Your help is greatly appreciated.
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