Rebecca Miller

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Questions related from Rebecca Miller

Western Blots for publication - to cut or not to cut?

Hi everyone, I am planning a series of Western Blot experiments where I will be probing for a range of targets in my samples. Whilst optimising, I have been cutting my membranes at the...

10 May 2023 6,820 3 View

Is there an effective method to increase the concentration of protein in cell lysate for Western Blotting?

My cell lysates are so dilute that to load 20ug of protein into my gel, the sample volume is too big for the wells. I have heard of using MWCO filters, and I have had suggestions to let the cell...

03 April 2023 9,759 4 View

Best supplier of human insulin for cell culture?

Hi everyone, I am looking to get some human insulin for cell culture work I have planned - I've ordered from Sigma but it won't get here till Christmas and I would rather get started sooner if...

02 November 2022 8,764 1 View

Does one 12well transwell insert provide enough protein cell lysate for WB?

I am looking to run westerns on cells that have grown on transwells for ~4months - do you have to pool multiple transwell inserts in order to have sufficient protein or is one transwell...

16 May 2022 1,421 2 View

Western Blots for publication - to cut or not to cut?

Hi everyone, I am planning a series of Western Blot experiments where I will be probing for a range of targets in my samples. Whilst optimising, I have been cutting my membranes at the relevant...

01 January 1970 9,740 5 View