What is the best way to sterilize silicone tubing in the context of my bioreactor needs?

07 August 2018 795 1 View

What is the minimum amount of time over which to use antibiotics/antimycotics in a primary cell culture?

When initiating a primary culture, what is the least amount of time antibiotics/antimycotics should be used? Would just a few hours be sufficient? In addition to issues affecting viability,...

31 December 2017 8,624 4 View

Is this a viable method of aseptic sampling from a bioreactor?

In brief, I want to sample media from a bioreactor on a daily basis, over a long period of time. The idea is to have an aspiration tube extending into the media, connected to a length of...

11 December 2017 1,368 3 View

Seal/Gasket sterility in bioreactors?

In bioreactors, obviously a significant location that presents a problem with the ingress of bacteria/contamination into the culture vessel is the areas around the seal/gaskets. I am wondering...

09 October 2017 7,850 1 View

Can I use a syringe filter for air?

09 October 2016 8,564 3 View

Can serum be separated from hemolymph?

07 August 2016 6,235 2 View

What is the best way to bulk-collect hemolymph?

07 August 2016 1,969 9 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View