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Questions related from Kessel Hedtsen
I’d like some advice regarding sterilizing a two-vessel bioreactor, specifically the tubing. The first vessel will be a media bottle and the second will be a custom-fabricated housing for a...
08 August 2018 860 1 View
When initiating a primary culture, what is the least amount of time antibiotics/antimycotics should be used? Would just a few hours be sufficient? In addition to issues affecting viability,...
01 January 2018 8,692 4 View
In brief, I want to sample media from a bioreactor on a daily basis, over a long period of time. The idea is to have an aspiration tube extending into the media, connected to a length of...
12 December 2017 1,439 3 View
In bioreactors, obviously a significant location that presents a problem with the ingress of bacteria/contamination into the culture vessel is the areas around the seal/gaskets. I am wondering...
10 October 2017 7,928 1 View
I am wondering if syringe filters, meant to be used with liquid, can be used with air. As a possible problem, I can see a dry filter not adhering to the housing properly. At times, the rate of...
10 October 2016 8,640 3 View
I have seen mention of using centrifugation in which tubes are stacked, with the top-most tube having a screen at the bottom to bulk-collect hemolymph. (Such a procedure is described in the link...
08 August 2016 2,104 9 View
As a general question regarding insects, mollusks, and crustaceans, I am wondering if non-clotting serum can be separated from hemolymph. In the case of mammalian blood, the method is pretty...
08 August 2016 6,306 2 View
This assumes fractional values of FBS no greater than 20 %. Is this problematic, in that there is fouling of equipment or cell death, or simply incidental? Thank you.
05 May 2016 5,963 1 View
