I have seen mention of using centrifugation in which tubes are stacked, with the top-most tube having a screen at the bottom to bulk-collect hemolymph. (Such a procedure is described in the link at the end of the post.)
What is the advantage of this method over simply using some sort of "press"? The only advantage that I can think of is that this method would mitigate cell lysis, and thus collection of cytosol in addition to hemolymph. Is this the case, or would a "press" work sufficiently? Are there any other methods that are commonly employed, perhaps with vacuum pumps, etc.?
Thank you.
Link:
http://brown.edu/Research/Fly_Aging/lab%20protocol/Hemolymph%20collection%20from%20adult%20flies.pdf
There is a method that might be useful to you in this paper. It is a "press" like technique.
Dr. Singh and Dr. Smith:
I don't see the papers that either of you are referring to. I see nothing in the body of the reply. When I clicked on Dr. Smith's profile, I saw a paper dealing with crustaceans that was clearly not the indicated paper.
Thanks.
Don't click on the link below of the reply text. There are two attachments below the body of each reply: 1.pdf and Baumberger and Olmsted 1928.pdf
Kessel,
Please give your mail id so that I can mail you the article
Sincerely
Hello Dear
The insect hemolymph can be collected by freezing anesthetized lepidopterous insects, piercing the epidermis of the frozen insects without damaging the alimentary canals thereof and then thawing them in a buffer solution containing a melanization-inhibitory agent to thus discharge the hemolymph of the insects into the buffer solution through the holes while making use of the self-contraction phenomenon caused during the thawing process.
Method for collecting hemolymph of insects
US 5866317 A
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