Are there any suspension cell lines for breast cancer? For example for cervical cancer we have HelaS3.

More Shivam Mishra's questions See All

Why do I see increased specs around the nucleus upon over-expression of cytoplasmic protein?

Upon over-expression of my protein through a piggyBAC-cometGFP vector, apart from seeing its cytoplasmic over-expression, I see some specs around the nucleus which I suppose is the localisation in...

31 December 2014 8,302 3 View

How do I remove IgG band covering my protein sample while doing a western blot for ChIP?

I am optimising ChIP for my protein which is approx. 45kDa in size. Upon doing Western Blot to check my pulldown I see a big IgG band that covers up my protein. Can anyone suggest me how to...

31 December 2014 1,837 5 View

I want to know if there is any publicly available SILAC repository that I can access to study the protein interaction network of any protein of interest?

Kindly suggest some references.

04 May 2013 1,732 0 View

How to perform ChIP on mouse testis with a small cell count?

I have isolated cells from a single mouse testis and assume that the number should be around 7-8 million. I want to do ChIP on them consisting of three immunoprecipitations (IPs). I need advice on...

04 May 2013 5,375 5 View

shRNA annealing and cloning

I have made repeated attempts to clone shRNA into pLKO vectors, but have not produced any colonies. I have tried annealing the shRNA in a thermocycler, in PCR, and in a water bath. For vector...

31 December 2012 2,735 16 View

Any tips for conducting ChIP with whole mouse testis?

I have to do Chromatin Immunoprecipitation (ChIP) with whole mouse testis. I have taken 6 mouse testis and I will be crosslinking with EGS and FA. Does anyone have any tips? Any tips particularly...

11 December 2012 4,214 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Radiogenomics Cancer Research Challenges?

what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...

03 August 2024 5,828 4 View