I have to do Chromatin Immunoprecipitation (ChIP) with whole mouse testis. I have taken 6 mouse testis and I will be crosslinking with EGS and FA. Does anyone have any tips? Any tips particularly for sonication conditions and pre-clearing?
I suggest you perform first preliminary experiments with different amount of testis tissue and different conditions for sonication (for example 60% -30% power, 4-6-8-10 strokes, always avoid to produce foam). Afterward check DNA sharing by running an agarose gel. If you see a smear between 200 ad 500bp your sample is good enough to proceed with cross-linking. If you perform ChIP assay the first time, test first your cross-linking conditions by performing ChIP with a control antibody like Histone 4.
Good luck Shivam,
Clara
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