Shivam Mishra

7 Questions 20 Answers 0 Followers

Questions related from Shivam Mishra

How do I remove IgG band covering my protein sample while doing a western blot for ChIP?

I am optimising ChIP for my protein which is approx. 45kDa in size. Upon doing Western Blot to check my pulldown I see a big IgG band that covers up my protein. Can anyone suggest me how to...

01 January 2015 1,798 5 View

Why do I see increased specs around the nucleus upon over-expression of cytoplasmic protein?

Upon over-expression of my protein through a piggyBAC-cometGFP vector, apart from seeing its cytoplasmic over-expression, I see some specs around the nucleus which I suppose is the localisation in...

01 January 2015 8,275 3 View

Are there any suspension cell lines for breast cancer? For example for cervical cancer we have HelaS3.

05 May 2013 6,151 2 View

I want to know if there is any publicly available SILAC repository that I can access to study the protein interaction network of any protein of interest?

Kindly suggest some references.

05 May 2013 1,709 0 View

How to perform ChIP on mouse testis with a small cell count?

I have isolated cells from a single mouse testis and assume that the number should be around 7-8 million. I want to do ChIP on them consisting of three immunoprecipitations (IPs). I need advice on...

05 May 2013 5,350 5 View

shRNA annealing and cloning

I have made repeated attempts to clone shRNA into pLKO vectors, but have not produced any colonies. I have tried annealing the shRNA in a thermocycler, in PCR, and in a water bath. For vector...

01 January 2013 2,689 16 View

Any tips for conducting ChIP with whole mouse testis?

I have to do Chromatin Immunoprecipitation (ChIP) with whole mouse testis. I have taken 6 mouse testis and I will be crosslinking with EGS and FA. Does anyone have any tips? Any tips particularly...

12 December 2012 4,191 1 View