Kindly suggest some references.
31 December 2014 8,224 3 View
I am optimising ChIP for my protein which is approx. 45kDa in size. Upon doing Western Blot to check my pulldown I see a big IgG band that covers up my protein. Can anyone suggest me how to...
31 December 2014 1,721 5 View
I have isolated cells from a single mouse testis and assume that the number should be around 7-8 million. I want to do ChIP on them consisting of three immunoprecipitations (IPs). I need advice on...
04 May 2013 5,301 5 View
Are there any suspension cell lines for breast cancer? For example for cervical cancer we have HelaS3.
04 May 2013 6,104 2 View
I have made repeated attempts to clone shRNA into pLKO vectors, but have not produced any colonies. I have tried annealing the shRNA in a thermocycler, in PCR, and in a water bath. For vector...
31 December 2012 2,610 16 View
I have to do Chromatin Immunoprecipitation (ChIP) with whole mouse testis. I have taken 6 mouse testis and I will be crosslinking with EGS and FA. Does anyone have any tips? Any tips particularly...
11 December 2012 4,143 1 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....
14 February 2021 1,602 4 View
Study bringing together genomics, proteomics etc together
02 February 2021 2,856 2 View
I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...
31 January 2021 3,405 3 View
Hi, I'm working with NanoLC/MS for both Metabolomics and Proteomics. We usually make our nano columns using emitters from NewObjective. Unfortunately New Objective, due to the pandemic, is now...
10 January 2021 1,176 4 View
Hi everyone, I am starting a project in analyzing EVs proteomics in cancers. However, the most published articles I have found stated only brief isolation protocols that could not fully be...
17 December 2020 7,600 2 View
Hi, I am trying to screen infected plant proteomes for targets of a secreted pathogen protein. I have purified the pathogen protein using a GST-tag on an agarose resin. I am using the Pierce...
23 November 2020 7,009 3 View
Hi, I want the MS2-CID spectra from orbitrap velos mass spectrometry of organism: human (Mod: C+57) for testing my peptide identification algorithm developed. Large set of orbitrap velos dataset...
16 November 2020 8,261 1 View
