Kindly suggest some references.
Upon over-expression of my protein through a piggyBAC-cometGFP vector, apart from seeing its cytoplasmic over-expression, I see some specs around the nucleus which I suppose is the localisation in...
31 December 2014 8,302 3 View
I am optimising ChIP for my protein which is approx. 45kDa in size. Upon doing Western Blot to check my pulldown I see a big IgG band that covers up my protein. Can anyone suggest me how to...
31 December 2014 1,837 5 View
I have isolated cells from a single mouse testis and assume that the number should be around 7-8 million. I want to do ChIP on them consisting of three immunoprecipitations (IPs). I need advice on...
04 May 2013 5,375 5 View
Are there any suspension cell lines for breast cancer? For example for cervical cancer we have HelaS3.
04 May 2013 6,183 2 View
I have made repeated attempts to clone shRNA into pLKO vectors, but have not produced any colonies. I have tried annealing the shRNA in a thermocycler, in PCR, and in a water bath. For vector...
31 December 2012 2,735 16 View
I have to do Chromatin Immunoprecipitation (ChIP) with whole mouse testis. I have taken 6 mouse testis and I will be crosslinking with EGS and FA. Does anyone have any tips? Any tips particularly...
11 December 2012 4,214 1 View
Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...
11 July 2024 8,484 0 View
I need some articles on the analysis of the nutritional quality of barley (H.vulgare.L) and the analytical approach of metabolomics and proteomics.
26 June 2024 3,451 2 View
Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...
04 June 2024 4,091 3 View
I have a proteomics dataset with missing values. I tried some strategies, but the point is that there are sets completely with missing values. The last strategy was to apply MissForest in python...
29 May 2024 5,563 4 View
We want to find interacting protein partners for our protein of interest.
28 April 2024 921 1 View
Due to factors outside of my control, peptides in my ESI-MS data have been ionised normally by protons ([M+2H], [M+3H]...) but also by sodium ([M+2Na], [M+3Na]...). Is there a way to configure...
16 April 2024 10,028 2 View
Hello, In the literature, there are some MS/MS results that include hypothetical proteins, which can be shorter than 40 amino acids. I can also find these when I search for an organism in the...
04 April 2024 5,098 2 View
How many ug/particles of EVs is needed for doing proteomics? We are doing many steps for purify EVs of human plasma and at the end of the process we are getting just 1/2ug/ml concentration of...
25 March 2024 730 1 View
serum proteomics
17 February 2024 1,086 3 View
Could this be due to an error in Mass Spec calibration or data analysis? I have 2 technical repeats that are fine, but the 3rd repeat is far away in the PCA plot and clusters with replicates of a...
05 February 2024 5,464 3 View
