The description on Sigma-Aldrich's website on DMEM Product Code D5671 states "Dulbecco’s Modified Eagle’s Medium - high glucose with 4500 mg/L glucose and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma". Does that imply that hybridoma cells are sensitive to L-glutamine and sodium pyruvate?
Edmund Pool
For hybridoma cultures you have to add glutamate and sodium pyruvate to medium just before medium change. Buy stock glutamax and sodium pyruvate from Sigma. Come as 50 or 100X stock solution. Store this in small aliquots at -20C. Add to medium as required.
1 votes
0 thanks
- Badges
- Science method
More Nurul Adhwa Rahman's questions See All
Similar questions and discussions