Can you identify this fungal growth (Fungi F)?

I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).

10 November 2015 4,084 26 View

Any advice on a cation-adjusted Mueller-Hinton broth?

10 November 2015 6,542 3 View

(Picture included) Can you help to identify this fungal growth (Fungi B)?

I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).

10 November 2015 4,648 2 View

(Picture included) Can you help to identify this fungal growth (Fungi C)?

I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB). Please note that there was a contamination of a...

10 November 2015 2,954 10 View

(Picture included) Can you help to identify this fungal growth (Fungi A)? Is this Penicillium sp.?

I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB). Because of the green growth, we are thinking...

10 November 2015 3,596 15 View

(Picture included) Can you help to identify this fungal growth (Fungi G)?

I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).

10 November 2015 8,430 7 View

How do I improvise DMEM to match a cell line's optimal growth condition?

08 September 2015 4,575 3 View

Alternative to 10% CO2 incubator?

My DMEM has 3.7g/L of Sodium bicarbonate, instead of 1.5g/L (I only have this DMEM), suggesting that my sensitive endothelial cells need 10% CO2 incubator. However I am working in an academic...

08 September 2015 8,273 6 View

DMEM changes colour to purple immediately upon addition of cells - What could have gone wrong?

I was reviving endothelial cells. When I added the cells to the DMEM, the medium immediately changes colour to purple. It was like a magic! Then one day after that, the medium returns back to red...

08 September 2015 1,725 11 View

Any experience with bEnd.3 cells?

Hello. My team has just revived bEnd.3 yesterday from a cryovial stored in liq nitrogen, purchased from ATCC, following the protocol stated by ATCC. After 24 hours, we only saw about 1-2 spindle...

07 August 2015 5,216 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View