I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).
With the images provided, you can only know that it is a dematiaceous fungus. The photomicrographs only show sterile hyphae. If you want to identify the organism at least to genus level, you need to induce sporulation. Probably the culture medium that you are using is too rich in nutrients.
You can try poor media, such as cornmeal agar, oatmeal agar, potato-carrot agar or simply water agar with sterilized plant material (for example, pine needles or carnation leaves). These media stimulate sporulation much better than more popular ones, such as Sabouraud agar.
Good luck with your identification!
Hugo
its not easy to identify the fungus from these pictures, please provide pictures for condiophore and conidia.
Good Luck
It's looks like Cladosporium on the media but we can't identify using this pictures you must have
You may check against Eurobasidium sp. However, the following references may be helpful:
Ellis MB. Dematiaceous Hyphomycetes. Key, Surrey: Commonwealth Mycological Institute, 1971.
Domsch et al. Compendium of Soil Fungi, Second Edition; 2007.
Regards
try to get growth and sporulation on other media. What is the source of the isolate?
As Hero M. Ismael said it is a dematiaceous fungus. Only hyphae are there but no spores. Grow the fungus on natural media like carrot, potatoe and potatoe-carrot agar for sporulation. Note that difficulties in research are the starting points for new discoveries.
i think it may be Xylariaceae- Daldinia Species.please provide another photo to show the fruiting body.
could you please give some picture about the condiophores and conida. This fungi should not be Cladosporium
Can you do ITS regions sequencing? It'll give you a glimpse into genus at least (at best).
It looks like Aureobasidium pullulans but cannot say for sure from the pictures.
The macro and microscopical features of this fungus give the probability of been CLADOSPORIUM CLADOSPORIODES .the MACROSCOPIC OLIVE COLOR OF FUNGAL COLONY is characteristic for cladosporium and may for alternaria sp. , the differential identification depend on the microscopical features of colonies .
see the attached ref.
With the images provided, you can only know that it is a dematiaceous fungus. The photomicrographs only show sterile hyphae. If you want to identify the organism at least to genus level, you need to induce sporulation. Probably the culture medium that you are using is too rich in nutrients.
You can try poor media, such as cornmeal agar, oatmeal agar, potato-carrot agar or simply water agar with sterilized plant material (for example, pine needles or carnation leaves). These media stimulate sporulation much better than more popular ones, such as Sabouraud agar.
Good luck with your identification!
Hugo
Usually Cladosporium easily makes conidiophores and conidia on several media, and you could see them (typical shape) with a binocular magnificent lens. Of course, dense black reverse is often typical of Cladosporium, but I'm not sure if in the worldly fungal diversity it couldn't exist anything else similar in shape. So, I agree with other colleagues: try to get fructification, and if it fails, sequence the ITS and compare to those in Genbank.
122
Hi
Yes, I agree with other colleagues,
You use a sequence the ITS and compare to those in Genbank, due to need some asexual structure, but you not show anything about the conidia or conidiophore
Thank you
its look like Cladosporium spp but there are no spore, you should stimulate it to produce spores.
Hi Nurul. Its look Dematiaceus fungi. I agree with other colleagues , yuo need do other test for more information, like molecular probes. Good luck with your identification.
Only on the basis of basis of fungal mycelium, one can not identify the fungus, There should be some spores. Try also PDA or Rose bengal with chloremphinicol for the culture.
Dear Franklin, The absence of a universally accepted DNA barcode for Fungi, is a serious limitation for ecological and taxonomic studies. Please check out the following article:
http://www.pnas.org/content/109/16/6241.full.pdf
Regards
yes is a dematiaceous fungus. Only hyphae are there but no spores. Grow the fungus on PDA or MEA media for sporulation.
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