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Questions related from Nurul Adhwa Rahman
I have Mueller-Hinton broth (CM0405, OXOID). However as stated in all CLSI documents, I should prepared it to be "cation-adjusted Mueller Hinton broth (CAMHB)" for fastidious bacteria. In the...
11 November 2015 6,687 3 View
I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB).
11 November 2015 4,732 2 View
11 November 2015 8,516 7 View
11 November 2015 9,472 3 View
I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB). Please note that there was a contamination of a...
11 November 2015 3,041 10 View
I have provided picture of the fungal growth on sabouraud agar, and its reverse morphology, also the staining using lactophenol cotton blue (LPCB). Because of the green growth, we are thinking...
11 November 2015 3,773 15 View
11 November 2015 4,179 26 View
I was reviving endothelial cells. When I added the cells to the DMEM, the medium immediately changes colour to purple. It was like a magic! Then one day after that, the medium returns back to red...
09 September 2015 1,814 11 View
My DMEM has 3.7g/L of Sodium bicarbonate, instead of 1.5g/L which is the optimal concentration for my cells. The problem is, I have already bought a lot of DMEM bottles! Is there any way that I...
09 September 2015 4,656 3 View
My DMEM has 3.7g/L of Sodium bicarbonate, instead of 1.5g/L (I only have this DMEM), suggesting that my sensitive endothelial cells need 10% CO2 incubator. However I am working in an academic...
09 September 2015 8,370 6 View
Hello. My team has just revived bEnd.3 yesterday from a cryovial stored in liq nitrogen, purchased from ATCC, following the protocol stated by ATCC. After 24 hours, we only saw about 1-2 spindle...
08 August 2015 5,315 3 View
Immunofluorescence staining is applied to cells either as in-vitro cell culture or in vivo animal models, and localisation of infectious agents is determined via the immunofluorescence...
06 June 2015 7,066 5 View
I am interested to understand the rationale behind the method of use (as mentioned in the file attached) as follows: 1. Why we cannot allow the suspension to sit at room temperature for longer...
06 June 2015 1,253 4 View
Has anyone ever made their own PTFE-coated microscope slides? The ready-made PTFE-coated microscope slides are sold at a high price in my country, so I am wondering if it is more cost-effective if...
05 May 2015 3,969 1 View
If the animals are to be either mice, mouse or rats, and the bacteria is to be Streptococcus pneumoniae. Are there any recommended books/paper on the health & safety guidelines on the...
04 April 2015 1,710 1 View
Have anyone worked with expired antibodies, of either the primary antibody or the conjugated secondary antibody (with HRP or fluorescein)? I know I could still work on few other expired chemicals...
03 March 2015 9,201 9 View
Hello everyone. Where do you think I could easily get Pseudomonas aeruginosa from the environment? I used to easily culture Vibrio parahaemolyticus from different shellfish on Vibrio chromogenic...
03 March 2015 4,539 17 View
The powdered crude plant extract could be stored at 4'C for how long? Could this be for 5-10 years? Are they any special precautions that I should be aware of in storing the extract?
03 March 2015 5,573 7 View
Have anyone tried different brands of non-fat dry milk for western blot, as blocking solution and antibody diluent? Would all brands work fine? I only know one Japanese brand that is widely used...
02 February 2015 2,856 5 View
When performing SDS PAGE, and then western blotting, do all the brands of markers/standards/ladder perform efficiently? Do you have a non-recommended marker based on your experience? I am...
02 February 2015 9,869 10 View
Mouse cell lines have the unique and natural ability to spontaneously transform and become immortalised, so that the cells can grow indefinitely in culture and maintained continuously. But how...
02 February 2015 7,384 2 View
The description on Sigma-Aldrich's website on DMEM Product Code D5671 states "Dulbecco’s Modified Eagle’s Medium - high glucose with 4500 mg/L glucose and sodium bicarbonate, without L-glutamine...
01 January 2015 6,793 2 View
If I were to use a clinical isolate of Streptococcus pneumoniae, without any information on the serotype, which antibody should I apply to view S. pneumoniae under the fluorescence microscopy? I...
12 December 2014 3,970 2 View
Which is better - to make my own SDS-PAGE gels according to the available recipe, or buy the commercialised ready-made SDS-PAGE gels? Will the performance of the ready-made gels be lower than the...
11 November 2014 8,794 17 View
I am interested to know why C6 is the famous cell line to be chosen to co-culture with bEnd.3 (endothelial cells).
11 November 2014 3,210 4 View
