Are the standards of modern research publications eroding?

True scholarly achievement resides in well-researched and cited publications. In our current Web 2.0 landscape, publishing has becoming easier and in turn, making it easier to publicly state and...

02 December 2020 7,392 7 View

How to do i replicate an insertion mutation in a cancer cell line?

I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I...

21 February 2020 3,918 2 View

Great project. Please could you share your reports for purposes of learning if project is completed. Wish to learn the crop losses estimation process?

Working on similar work.

16 January 2019 2,207 3 View

Are there accepted and published standards for DC microgrids?

Dear all, Please, are there any standards for DC microgrids? For example, what should be the maximum percentage of DC bus voltage transient dip, the accepted duration of a transient etc.

20 November 2018 3,126 2 View

Who has and knows comprehensively how to use SPSS VERSION 20?

It a statistical tool for data analysis.

14 April 2018 1,993 5 View

How do you determine sample size for an unknown population?

I am trying to determine the sample size for an ongoing study on traditional authority. I need to determine the sample size for the population of people who have dealt with traditional authority...

20 December 2017 9,192 12 View

Collaborative research on investigating changes of cannabis flower throughout curing?

21 May 2017 4,620 2 View

At what specific temperature is suitable for drying alpha cellulose to prevent it from browning?

Is it good for normal room temperature where perforated cloth is used to cover it to prevent any fallen objects into the sample? Drying in the oven at what temperature and the time for drying

02 March 2016 5,805 2 View

How can i dry alpha cellulose without being turning to brown?

The specific temperature is required The mode of drying is required

29 February 2016 3,992 3 View

Can someone suggest a company or a group that can produce monodispersed spherical GNPs?

We are looking for a company or a group that can make for us monodispersed spherical Gold nanoparticles 250nm with higher number of GNPs per milliliter with orders of x10E12 particles per ml or...

19 December 2013 4,466 2 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View