Are the standards of modern research publications eroding?

True scholarly achievement resides in well-researched and cited publications. In our current Web 2.0 landscape, publishing has becoming easier and in turn, making it easier to publicly state and...

02 December 2020 7,392 7 View

How to do i replicate an insertion mutation in a cancer cell line?

I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I...

21 February 2020 3,918 2 View

Who has and knows comprehensively how to use SPSS VERSION 20?

It a statistical tool for data analysis.

14 April 2018 1,993 5 View

How to model circular pile in plain strain

I have a row of 6 circular pile spaced at 4m.Each pile is suppose to carry 400kN.I want to model this piles as an equivalent wall.How do I get the equivalent wall thickness and the equivalent load...

05 June 2017 1,618 1 View

How to model circular pile in plain strain

I have a row of 6 circular pile spaced at 4m.Each pile is suppose to carry 400kN.I want to model this piles as an equivalent wall.How do I get the equivalent wall thickness and the equivalent load...

05 June 2017 2,061 1 View

Collaborative research on investigating changes of cannabis flower throughout curing?

21 May 2017 4,620 2 View

Factors that controls tunnel lining forces?

I am trying to compare lining forces for tunnels in shanghai soft clay,Bangkok soft clay,Boom clay and Lateritic clay(latosol).What parameters of clay soils controls tunnel lining forces?

26 April 2017 1,890 1 View

Factors that controls tunnel lining forces?

I am trying to compare lining forces for tunnels in shanghai soft clay,Bangkok soft clay,Boom clay and Lateritic clay(latosol).What parameters of clay soils controls tunnel lining forces?

03 April 2017 9,727 1 View

Stress reduction factors?

I am simulating a shallow tunnel in plaxis 2D using the stress reduction method.The Tunnel diameter is 6m with an overburden of 7.6m.what Stress reduction value can be applied?

03 April 2017 3,621 5 View

Apart from BGM what other cells can I used for TCID for polio virus?

Apart from BGM what other cells can I use for TCID for polio virus?

13 August 2015 4,234 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View