After CTAB extraction I got 210 ng of DNA concentration and the ratio values were less than 1.7 (260/280) and less than 1.8 at 260/230 ration.
Leishmania
02 May 2023 3,709 3 View
I received a peer-review invitation from Qeios. Is anyone familiar with Qeios? Is Qeios a genuine publisher?
13 November 2022 4,418 17 View
By the policy, all vocational programs at the Senior High School are practical oriented programs. Therefore, students who after going through such programs should be able to acquire the...
11 February 2021 8,991 7 View
True scholarly achievement resides in well-researched and cited publications. In our current Web 2.0 landscape, publishing has becoming easier and in turn, making it easier to publicly state and...
02 December 2020 7,556 7 View
I am a phd student. I do my rechearch in combustio process in a traditional kiln
12 July 2020 8,667 1 View
I'm running an AIN/AllnN DBR simulation in the UV region and designed a pattern in the UV A, B, and C regions. My challenge is finding ways to see the patterns in the different UV regions.
14 March 2020 9,131 2 View
I carried out a WGS of the mitochondria DNA in cancer and its corresponding adjacent tissues. The analysis show two significant SNPs with a very high OR of exposure to cancer. Consequently, I...
21 February 2020 4,056 2 View
It a statistical tool for data analysis.
14 April 2018 2,146 5 View
I've attempted H. bacteriophora, S. feltiae, and S. carpocapsae with failure. Any ideas on other species? Thanks, Allison
10 April 2018 4,478 4 View
I am trying to look for the best method for plasm eccDNA, i may want to assess copy number variations in plasma eccDNA, but seems the yield will be little without a specific enrichment procedure...
28 November 2017 9,236 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View