Why am I not getting colonies using the Agilent QuikChange II kit?

Hello everyone, I hope you are very well. I am performing site-directed mutations with the QuikChange II kit. My first ones have a length of 50bp and a Tm of between 75 and 81 approximately. I...

09 November 2020 242 2 View

Exist some reliable website with information about seric calcium and phosphorus in Chelonoidis chilensis?

Someone will have papers, books or magazine articles about it. It's for my thesis. Thanks

03 June 2019 9,730 2 View

Great project. Please could you share your reports for purposes of learning if project is completed. Wish to learn the crop losses estimation process?

Working on similar work.

16 January 2019 2,207 3 View

Are there accepted and published standards for DC microgrids?

Dear all, Please, are there any standards for DC microgrids? For example, what should be the maximum percentage of DC bus voltage transient dip, the accepted duration of a transient etc.

20 November 2018 3,126 2 View

How do you determine sample size for an unknown population?

I am trying to determine the sample size for an ongoing study on traditional authority. I need to determine the sample size for the population of people who have dealt with traditional authority...

20 December 2017 9,192 12 View

Which one is the best protocol or extraction step for RNA extraction in 3T3-L1 cell line?

I used the standard protocol for this, but the lipids still there. they should be in the top but it doesn't appear, so when I use the chloroform the lipids are in the top of the organic phase

11 December 2016 2,775 10 View

Which (python) workflow could I use to manage meteorological data?

07 June 2016 6,150 5 View

At what specific temperature is suitable for drying alpha cellulose to prevent it from browning?

Is it good for normal room temperature where perforated cloth is used to cover it to prevent any fallen objects into the sample? Drying in the oven at what temperature and the time for drying

02 March 2016 5,805 2 View

How can i dry alpha cellulose without being turning to brown?

The specific temperature is required The mode of drying is required

29 February 2016 3,992 3 View

Apart from BGM what other cells can I used for TCID for polio virus?

Apart from BGM what other cells can I use for TCID for polio virus?

13 August 2015 4,234 1 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Is anyone working on a project focused on immigrants/newcomers working in the hospitality industry and how they have been impacted by COVID?

The on-going Covid-19 pandemic has had devastating economic effects on many industries. However, few have suffered to the same extent as full-service restaurants. Numerous studies have shown that...

24 February 2021 9,496 4 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View