Is the quality of our RNA sufficient for RNA sequencing?

Hi, We are checking the integrity of RNA isolated (using MagMAX 96 Total RNA Isolation kit) from mice tissue on a 1% agarose gel that contains a bit of bleach to get rid of RNases. Our 28S/18S...

23 November 2020 5,006 3 View

How do I give glucose solution to rats wistar after STZ intra-peritoneal dose to prevent hypoglycemic shock?

How to administer the 10% glucose dose in the rats after the STZ dose? Is the solution placed in the drinkers or is it given intragastrically? if it is intragastric, in how many ml to dissolve the...

19 July 2020 4,178 2 View

Book for demographic modelling?

Dear all, can anyone recommend a good introduction to demographic modelling in population genetics? I am mainly interested in books but paper suggestions are also welcome.Thanks a lot! Best...

10 June 2020 8,626 2 View

Sources to purchase purified Echinochrome A extract?

I am looking to make an Echinochrome A calibration curve for microplate visual spectral analysis of S. purpuratus coelomic fluid.

02 October 2019 8,955 1 View

No p-value for Mauchly's Test of Sphericity in SPSS?

Hi all, I've had the same conflict with SPSS not providing a p-values for Mauchly's Test of Sphericity; this occurred for two different 3-WAY-RM-ANOVAs I recently ran. In one of the...

12 August 2018 3,811 4 View

What causes a failure of read error correction in Quorum (MASURCA)?

Dear all, I am currently trying to run the MASURCA assembler with my Illumina paired-end reads. The first steps work fine but when it comes to the error correction with Quorum the program reports...

13 July 2016 7,629 2 View

Extraction from bee museum specimens?

Dear all, I aim to amplify several nuclear genes (500-1100 bp) for dried museum specimens of the bee genus Rediviva (family: Melittidae). We got one middle leg per specimen for extraction...

29 June 2016 6,792 6 View

What is the minimum sample size for population genetic study using SNPS?

I'm planning to do a population genetic analyses (genetic differentiation, diversity) on a bee species using SNP markers. Furthermore, I aim to identify genes under selection. For population...

06 May 2014 9,250 10 View

The quirky nature of neuropeptides

Reading this enjoyable review by Anthony van den Pol about neuropeptides, wondering if you are investigating peptides too, and what fascinates you about them? For example, I am fascinated about...

02 July 2013 2,489 1 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View