Is the quality of our RNA sufficient for RNA sequencing?

Hi, We are checking the integrity of RNA isolated (using MagMAX 96 Total RNA Isolation kit) from mice tissue on a 1% agarose gel that contains a bit of bleach to get rid of RNases. Our 28S/18S...

23 November 2020 5,006 3 View

Free database for auditory stimuli?

Hi, I'm currently looking for auditory stimuli (list of letters; word/non-words; etc) to include as distraction task in my experiment. Does anybody know a free database that I might download?...

04 October 2020 9,614 3 View

How do I give glucose solution to rats wistar after STZ intra-peritoneal dose to prevent hypoglycemic shock?

How to administer the 10% glucose dose in the rats after the STZ dose? Is the solution placed in the drinkers or is it given intragastrically? if it is intragastric, in how many ml to dissolve the...

19 July 2020 4,178 2 View

Book for demographic modelling?

Dear all, can anyone recommend a good introduction to demographic modelling in population genetics? I am mainly interested in books but paper suggestions are also welcome.Thanks a lot! Best...

10 June 2020 8,626 2 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

DGGE. Does anyone have had problems with the separation of fungal bands on acrylamide gels?

24 October 2018 8,251 6 View

DGGE. Does anyone have had problems with the separation of fungal bands on acrylamide gels?

09 October 2018 8,651 7 View

What causes a failure of read error correction in Quorum (MASURCA)?

Dear all, I am currently trying to run the MASURCA assembler with my Illumina paired-end reads. The first steps work fine but when it comes to the error correction with Quorum the program reports...

13 July 2016 7,629 2 View

Extraction from bee museum specimens?

Dear all, I aim to amplify several nuclear genes (500-1100 bp) for dried museum specimens of the bee genus Rediviva (family: Melittidae). We got one middle leg per specimen for extraction...

29 June 2016 6,792 6 View

What kind of measurement scales can I use in a questionnaire, when the statistical technique is PLS?

In my research model the independent variables have questions Likert scale , while the dependent variables are dichotomous questions; with PLS Can I combine these types of questions?

30 October 2015 3,282 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View