We have been making viruses using HIV packaging system for years in our lab. We use 3rd generation system and make virus in 293T cells. We have been adding specific mutations to envelope proteins and one particular version of the envelope protein is not expressed well in virions (used a western to confirm) , whereas p24 levels (WB) and RT assay show comparable titer to wild type envelope protein. Any ideas if it is the cells or the mutations in the envelope affecting the envelope protein expression in virions?
These “naked” viruses only need their protein-based capsid and host detector proteins to infect host cells. However, because they lack a lipid envelope, they more resistant to many disinfectants and other stresses like drying out or heat . The packaging of host proteins (e.g., the ... Furthermore, non-HIV viral ... Env glycoprotein incorporation into ... of the gp41 mutant virus in culture. ... The mechanism responsible for the altered Gag and ... in a mammalian two-hybrid system.
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